Recombinant Human Follistatin 300 Protein

Carrier Free

Catalog # Availability Size / Price Qty
669-FO-025/CF

With Carrier

Catalog # Availability Size / Price Qty
669-FO-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (16)
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Recombinant Human Follistatin 300 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL of rhActivin A.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Follistatin protein
Gly30-Asp329, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
31 kDa
SDS-PAGE
38-43 kDa, reducing conditions

Product Datasheets

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669-FO (with carrier)

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669-FO/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

669-FO

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

669-FO/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS‑288) and 315 aa residues (FS‑315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS‑315 composed of 300 aa residues. The recombinant human FS‑300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS‑288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS‑315 binds with low‑affinity. The binding affinity of R&D Systems' FS‑300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Over‑expression of FS can also cause reproductive defects in transgenic mice.

References
  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Citations for Recombinant Human Follistatin 300 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. An autocrine ActivinB mechanism drives TGF?/Activin signaling in Group 3 medulloblastoma
    Authors: M Morabito, M Larcher, FM Cavalli, C Foray, A Forget, L Mirabal-Or, M Andrianter, S Druillenne, A Garancher, J Masliah-Pl, S Leboucher, A Debalkew, A Raso, O Delattre, S Puget, F Doz, MD Taylor, O Ayrault, F Bourdeaut, A Eychène, C Pouponnot
    EMBO Mol Med, 2019;0(0):e9830.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats
    Authors: J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang
    Mol Med Rep, 2018;17(4):5074-5080.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages
    Authors: J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao
    Cell Transplant, 2018;0(0):9636897187803.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Functional role of AKT signaling in bovine early embryonic development: potential link to embryotrophic actions of follistatin
    Authors: M Ashry, SK Rajput, JK Folger, JG Knott, NA Hemeida, OM Kandil, RS Ragab, GW Smith
    Reprod. Biol. Endocrinol., 2018;16(1):1.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis
    Authors: A Jørgensen, J Macdonald, JE Nielsen, KR Kilcoyne, S Perlman, L Lundvall, L Langhoff T, K Juul Hare, H Frederikse, AM Andersson, NE Skakkebæk, A Juul, RM Sharpe, E Rajpert-De, RT Mitchell
    Cell Rep, 2018;25(7):1924-1937.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion
    Authors: P De Berdt, P Bottemanne, J Bianco, M Alhouayek, A Diogenes, A Llyod, J Gerardo-Na, GA Brook, V Miron, GG Muccioli, AD Rieux
    Cell. Mol. Life Sci., 2018;0(0):.
    Species: Human/Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer
    Authors: DD Seachrist, ST Sizemore, E Johnson, FW Abdul-Kari, KL Weber Bonk, RA Keri
    Breast Cancer Res., 2017;19(1):66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
    Authors: Jonas J Staudacher, Cemal Yazici, Timothy Carroll, Jessica Bauer, Jingbo Pang, Nancy Krett, Yinglin Xia, Annette Wilson, Georgios Papachris, Andrea Dirmeier, Claudia Kunst, David C Whitcomb, Giamila Fantuzzi, Barbara Jung
    Scientific Reports, 2017;0(0):2045-2322.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Involvement of Infiltrating Macrophage-derived Activin A in the Progression of Renal Damage in MRL-lpr Mice
    Authors: Yoshihisa Nojima
    Am. J. Physiol. Renal Physiol., 2016;0(0):ajprenal.0019.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB.
    Authors: Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J
    Arthritis Rheum., 2007;56(11):3715-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin.
    Authors: Wen Z, Han L, Bamburg JR, Shim S, Ming GL, Zheng JQ
    J. Cell Biol., 2007;178(1):107-19.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay
  14. The activin-follistatin system and in vitro early follicle development in goats.
    Authors: Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R
    J. Endocrinol., 2006;189(1):113-25.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: Bioassay
  15. Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis.
    Authors: Amthor H, Nicholas G, McKinnell I, Kemp CF, Sharma M, Kambadur R, Patel K
    Dev. Biol., 2004;270(1):19-30.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  16. Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
    Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
    J. Bone Miner. Res., 2004;19(8):1302-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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