Recombinant Human Galactosylceramidase/GALC Protein, CF

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Recombinant Human Galactosylceramidase/GALC Protein Enzyme Activity Diagram.
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Product Details
Citations (3)
Reviews (1)

Recombinant Human Galactosylceramidase/GALC Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The specific activity is >550 pmol/min/ug, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human Galactosylceramidase/GALC protein
Met1-Arg685, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
74 kDa
70-85 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM Sodium Citrate, 125 mM NaCl, 0.5% Triton® X-100, pH 4.5
  • Stop Solution: 0.5 M Glycine, 0.3 M NaOH (~pH 10.0)
  • Recombinant Human Galactosylceramidase/GALC (rhGALC) (Catalog # 7310-GH)
  • Substrate: 4-methylumbelliferyl-beta -D-galactopyranoside (Sigma, Catalog # M1633), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhGALC to 0.06 ng/μL in Assay Buffer.
  2. Dilute Substrate to 1 mM in Assay Buffer.
  3. Load in a plate 25 μL of 0.06 ng/μL rhGALC, and start the reaction by adding 25 μL of 1 mM Substrate.  Include a Substrate Blank containing 25 μL of Assay Buffer and 25 μL of 1 mM Substrate.
  4. Seal plate and incubate at 37 °C for 20 minutes.
  5. After incubation, stop the reactions by adding 50 μL of Stop Solution to each well.
  6. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in endpoint mode.
  7. Calculate specific activity: 

     Specific Activity (pmol/min/µg) =

Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 4-methylumbelliferone (Sigma, Catalog # M1381).

Per Well:
  • rhGALC: 0.0015 μg
  • Substrate: 0.25 mM

Scientific Data

Enzyme Activity View Larger

Recombinant Human Galactosylceramidase/GALC Protein Enzyme Activity Diagram. Recombinant Human Galactosylceramidase/GALC Protein, CF (Catalog # 7310-GH) catalyzes the hydrolysis of beta -d-galactocerebroside to beta -d-galactose and ceramide.

Enzyme Activity View Larger

Recombinant Human Galactosylceramidase/GALC Protein, CF (Catalog # 7310-GH) is measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Galactosylceramidase/GALC Protein (Catalog # 7310-GH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-85 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Galactosylceramidase/GALC

Galactosylceramidase (GALC or galactocerebrosidase) is a lysosomal enzyme that hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride, molecules that are important in the production of normal myelin (1). Mutations in the GALC gene have been associated with Krabbe’s Disease, also known as globoid cell leukodystrophy (2, 3, 4), a fatal autosomal recessive disorder resulting from the insufficient catabolism of these galactolipids. Krabbe’s Disease is morphologically characterized by almost total absence of myelin, severe gliosis, and the presence of characteristic multinucleated globoid cells in the white matter (4). In a recent study, cord blood transplants have been successful in stopping the disease (5).

  1. Chen, Y.Q. et al. (1993) Hum. Mol. Genet. 2:1841.
  2. Tappino, B. et al. (2010) Hum. Mutat. 31:E1894.
  3. Kodama, S. et al. (1982) J Neurochem. 39:1314.
  4. Suzuki, K. and Suzuki, Y. (1970) Proc. Natl. Acad. Sci. U.S.A. 66:302.
  5. Escolar, M.L. et al. (2005) N. Engl. J. Med. 352:2069.
Entrez Gene IDs
2581 (Human); 14420 (Mouse); 314360 (Rat)
Alternate Names
EC; Galactocerebrosidase; Galactocerebroside beta-galactosidase; galactosylceramidase (Krabbe disease); Galactosylceramidase; Galactosylceramide beta-galactosidase; Galactosylceraminidase; GALC; GALCERase

Citations for Recombinant Human Galactosylceramidase/GALC Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Substrate reduction therapy for Krabbe disease and metachromatic leukodystrophy using a novel ceramide galactosyltransferase inhibitor
    Authors: MC Babcock, CR Mikulka, B Wang, S Chandriani, S Chandra, Y Xu, K Webster, Y Feng, HR Nelvagal, A Giaramita, BK Yip, M Lo, X Jiang, Q Chao, JC Woloszynek, Y Shen, S Bhagwat, MS Sands, BE Crawford
    Scientific Reports, 2021;11(1):14486.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Fluorimetric assay with a novel substrate for quantification of galactocerebrosidase activity in dried blood spot specimens
    Authors: AJ Ullal, H Pham, R Singh, P Ross, CA Graham, SM Norton, MH Nuffer, DS Burns, AE Eckhardt, M Escolar, D Bali, VK Pamula
    Pract Lab Med, 2020;18(0):e00141.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly identified brain sterol metabolite, galactosylated cholesterol
    Authors: H Akiyama, M Ide, Y Nagatsuka, T Sayano, E Nakanishi, N Uemura, K Yuyama, Y Yamaguchi, H Kamiguchi, R Takahashi, JMFG Aerts, P Greimel, Y Hirabayash
    J. Biol. Chem., 2020;0(0):.
    Applications: Bioassay


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Recombinant Human Galactosylceramidase/GALC Protein, CF
By Anonymous on 05/23/2016
Application: Enzymatic activity in vitro