Recombinant Human Galectin-3BP/MAC-2BP Protein, CF

Catalog # Availability Size / Price Qty
2226-GAB-050
Recombinant Human Galectin-3BP/MAC-2BP Protein Bioactivity
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Product Details
Citations (4)
FAQs
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Recombinant Human Galectin-3BP/MAC-2BP Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-3BP/MAC-2BP is immobilizedat 0.5 µg/mL,100 μL/well, It can bind Recombinant Human Galectin‑3 (Catalog # 1154-GA) with an ED50 of 0.2-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Galectin-3BP/MAC-2BP protein
Val19-Asp585, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Val19
Predicted Molecular Mass
65 kDa
SDS-PAGE
26-30 kDa, 60-69 kDa and 81-94 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2226-GAB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Galectin-3BP/MAC-2BP Protein Bioactivity View Larger

When Recombinant Human Galectin-3BP/MAC-2BP (Catalog # 2226-GBA) is immobilized at 0.5 µg/mL, 100 µL/well, It binds Recombinant Human Galectin-3 (Catalog # 1154-GA) with an ED50 of 0.2-1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell‑rich tissues (1‑3). The 585 amino acid (aa) human Galectin‑3BP contains an 18 aa signal sequence and four definitive domains (4‑6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid‑phase adhesion profile of full‑length Galectin‑3BP (5, 6). Glycosylation at seven N‑linked sites, generates a molecular size of 85‑97 kDa (1, 2, 6). Galectin‑3BP dimers form linear and ring‑shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin‑3BP has been shown to stimulate natural killer cells and lymphokine‑activated killer cell activity (2). High Galectin‑3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin‑3BP shares 69% aa identity with mouse cyclophilin C‑associated protein (CyCAP), which does not appear to bind Galectin‑3 (8). Human Galectin‑3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N‑terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported (9).

References
  1. Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
  2. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
  3. Sasaki, T. et al. (1998) EMBO J. 17:1606.
  4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
  5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
  6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
  7. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
  8. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
  9. Entrez Accession # EAW89544.
Long Name
Galectin-3 Binding Protein
Entrez Gene IDs
3959 (Human)
Alternate Names
90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K

Citations for Recombinant Human Galectin-3BP/MAC-2BP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Galectin 3-binding protein suppresses amyloid-&beta production by modulating &beta-cleavage of amyloid precursor protein
    Authors: T Seki, M Kanagawa, K Kobayashi, H Kowa, N Yahata, K Maruyama, N Iwata, H Inoue, T Toda
    J. Biol. Chem., 2020;295(11):3678-3691.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Galectin-3 Binding Protein Secreted by Breast Cancer Cells Inhibits Monocyte-Derived Fibrocyte Differentiation.
    Authors: White M, Roife D, Gomer R
    J Immunol, 2015;195(4):1858-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
    Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
    Cancer Res, 2012;72(9):2228-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
    Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
    J. Biol. Chem., 2008;283(27):18573-81.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

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