Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
714-GR-100
Product Details
Citations (9)
FAQs
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Recombinant Human GFR alpha-1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF (Catalog # 212-GD) at 1 µg/mL binds Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent Kd <2 nM.
Source
Mouse myeloma cell line, NS0-derived human GFR alpha-1/GDNF R alpha-1 protein
Human GFR alpha -1
(Ala19-Lys429)
Accession # NP_665736
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
73.3 kDa (monomer)
SDS-PAGE
88-95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

714-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta  superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane‑associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
Entrez Gene IDs
2674 (Human); 14585 (Mouse); 25454 (Rat)
Alternate Names
DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546

Citations for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. RET signaling in prostate cancer
    Authors: K Ban, S Feng, L Shao, M Ittmann
    Clin. Cancer Res., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Parkin cooperates with GDNF/RET signaling to prevent dopaminergic neuron degeneration.
    Authors: Meka D, Muller-Rischart A, Nidadavolu P, Mohammadi B, Motori E, Ponna S, Aboutalebi H, Bassal M, Annamneedi A, Finckh B, Miesbauer M, Rotermund N, Lohr C, Tatzelt J, Winklhofer K, Kramer E
    J Clin Invest, 2015;125(5):1873-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants.
    Authors: Klein P, Muller-Rischart A, Motori E, Schonbauer C, Schnorrer F, Winklhofer K, Klein R
    EMBO J, 2014;33(4):341-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. RET PLC-gamma phosphotyrosine binding domain regulates Ca2+ signaling and neocortical neuronal migration.
    Authors: Lundgren TK, Nakahata K, Fritz N, Rebellato P, Zhang S, Uhlen P
    PLoS ONE, 2012;7(2):e31258.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP
  7. Neurturin-GFRalpha2 signaling controls liver bud migration along the ductus venosus in the chick embryo.
    Authors: Tatsumi N, Miki R, Katsu K, Yokouchi Y
    Dev. Biol., 2007;307(1):14-28.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. A specific survival response in dopamine neurons at most risk in Parkinson's disease.
    Authors: Murase S, McKay RD
    J. Neurosci., 2006;26(38):9750-60.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
    Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
    Biochemistry, 2005;44(7):2545-54.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: ELISA Developmet

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