Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Recombinant Human GDNF Protein
R&D Systems | Catalog # 212-GD
Key Product Details
- R&D Systems NS0-derived Recombinant Human GDNF Protein (212-GD)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Arg109-Ile211
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
Reviewed Applications
Read 4 reviews rated 4.8 using 212-GD in the following applications:
Scientific Data Images for Recombinant Human GDNF Protein
Recombinant Human GDNF Protein SEC-MALS.
Recombinant human GDNF (Catalog # 212-GD) has a molecular weight (MW) of 27.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human GDNF Protein Bioactivity
Recombinant Human GDNF (Catalog # 212-GD) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFRa-1 Fc Chimera (Catalog # 714-GR). The activity is approximately 9-fold greater than the top competitor's GDNF.
Recombinant Human GDNF Protein SDS-PAGE
1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 212-GD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100-200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 212-GD/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100-200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: GDNF
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human GDNF Protein
For research use only
Related Research Areas
Citations for Recombinant Human GDNF Protein
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Customer Reviews for Recombinant Human GDNF Protein (4)
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Customer Images
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 06/22/2020
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Application: Immunoassay StandardVerified Customer | Posted 07/19/2017Used for control recovery in the Duo-set GDNF elisa and found that recovery was withing limit
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 05/26/2017
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 08/01/2016
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