Recombinant Human GPIHBP1 Fc Chimera Protein, CF

R&D Systems | Catalog # 10091-GB

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human GPIHBP1 Fc Chimera Protein (10091-GB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q8IV16

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GPIHBP1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GPIHBP1 protein
Human GPIHBP1
(Thr22-Gly151)
Accession # Q8IV16		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr22

Predicted Molecular Mass

41 kDa

SDS-PAGE

52-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human GPIHBP1 Fc Chimera that produces 50% optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

Recombinant Human GPIHBP1 Fc Chimera Protein Binding Activity

Recombinant Human GPIHBP1 Fc Chimera Protein Binding Activity

When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 µg/mL,100 µL/well, Recombinant Human GPIHBP1 Fc Chimera (Catalog # 10091-GB) binds with an ED50 of 1.5-9 µg/mL.
Recombinant Human GPIHBP1 Fc Chimera Protein SDS-PAGE

Recombinant Human GPIHBP1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human GPIHBP1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-63 kDa and 100-130 kDa, respectively.

Formulation, Preparation, and Storage

10091-GB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPIHBP1

GPIHBP1 (Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1) is a member of the Ly6 family of proteins (1, 2). Human GPIHBP1 is synthesized as a 184 amino acid (aa) protein that includes a 20 aa signal peptide, a 131 aa GPIHBP1 chain containing the Ly6 domain, and a 33 aa propeptide. Within the main chain region, human GPIHBP1 shares 54% and 53% aa sequence identity with mouse and rat GPIHBP1, respectively. It is found on capillaries of heart, skeletal muscle, and adipose tissue (3), but not expressed in endothelial cells of larger blood vessels or expressed in capillaries of the brain (3, 4).  GPIHBP1 binds both lipoprotein lipase (LPL) and chylomicrons (CM), and the acidic domain of GPIHBP1 is required for LPL binding (3, 5). Triglyceride-rich lipoproteins undergo lipolysis by LPL bound by GPIHBP1 and transported across the cells to the capillary lumen (6-7). GPIHBP1 is an important regulator of triglyceride metabolism by increasing the efficiency of lydrolysis by LPL and uptake of fatty acids (3, 8-9).

References

  1. Young, S.G. et al. (2011) J. Lipid Res. 52:1869.
  2. Ioka, R.X. et al. (2003) J. Biol. Chem. 278:7344.
  3. Beigneux, A.P. et al. (2007). Cell Metab. 5:279.
  4. Davies, B.S.J. et al. (2010) Cell Metab. 12:42.
  5. Gin, P. (2008) J. Biol. Chem. 283:29554.
  6. Davies, B.S. et al. (2012) J. Lipid Res. 53:2690.
  7. Goulborne, C.N. et al. 2014 Cell Metabolism 19:849.
  8. Young, S.G. et al. (2007) Curr. Opin. Lipidol. 18:389.
  9. Sonneburg, W.K. et al. (2009) J Lipid Research 50:2421.

Long Name

Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1

Alternate Names

GPI-Anchored HDL-Binding Protein 1, GPI-HBP1, HBP1, HYPL1D

Entrez Gene IDs

338328 (Human); 68453 (Mouse); 300027 (Rat)

Gene Symbol

GPIHBP1

UniProt

Q8IV16

Additional GPIHBP1 Products

Product Documents for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

For research use only

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