Recombinant Human GPIHBP1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10091-GB-050
Recombinant Human GPIHBP1 Fc Chimera Protein, CF Binding Activity
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Recombinant Human GPIHBP1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human GPIHBP1 Fc Chimera that produces 50% optimal binding response is 1.5-9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human GPIHBP1 protein
Human GPIHBP1
(Thr22-Gly151)
Accession # Q8IV16
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Thr22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
52-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10091-GB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 μg/mL,100 μL/well, Recombinant Human GPIHBP1 Fc Chimera (Catalog # 10091-GB) binds with an ED50 of 1.5-9 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human GPIHBP1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 52-63 kDa and 100-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPIHBP1

GPIHBP1 (Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1) is a member of the Ly6 family of proteins (1, 2). Human GPIHBP1 is synthesized as a 184 amino acid (aa) protein that includes a 20 aa signal peptide, a 131 aa GPIHBP1 chain containing the Ly6 domain, and a 33 aa propeptide. Within the main chain region, human GPIHBP1 shares 54% and 53% aa sequence identity with mouse and rat GPIHBP1, respectively. It is found on capillaries of heart, skeletal muscle, and adipose tissue (3), but not expressed in endothelial cells of larger blood vessels or expressed in capillaries of the brain (3, 4).  GPIHBP1 binds both lipoprotein lipase (LPL) and chylomicrons (CM), and the acidic domain of GPIHBP1 is required for LPL binding (3, 5). Triglyceride-rich lipoproteins undergo lipolysis by LPL bound by GPIHBP1 and transported across the cells to the capillary lumen (6-7). GPIHBP1 is an important regulator of triglyceride metabolism by increasing the efficiency of lydrolysis by LPL and uptake of fatty acids (3, 8-9).

References
  1. Young, S.G. et al. (2011) J. Lipid Res. 52:1869.
  2. Ioka, R.X. et al. (2003) J. Biol. Chem. 278:7344.
  3. Beigneux, A.P. et al. (2007). Cell Metab. 5:279.
  4. Davies, B.S.J. et al. (2010) Cell Metab. 12:42.
  5. Gin, P. (2008) J. Biol. Chem. 283:29554.
  6. Davies, B.S. et al. (2012) J. Lipid Res. 53:2690.
  7. Goulborne, C.N. et al. 2014 Cell Metabolism 19:849.
  8. Young, S.G. et al. (2007) Curr. Opin. Lipidol. 18:389.
  9. Sonneburg, W.K. et al. (2009) J Lipid Research 50:2421.
Long Name
Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1
Entrez Gene IDs
338328 (Human); 68453 (Mouse); 300027 (Rat)
Alternate Names
glycosylphosphatidylinositol anchored high density lipoprotein binding protein1; glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein1; GPI anchored high density lipoprotein binding protein 1; GPI-Anchored HDL-Binding Protein 1; GPIHBP1; GPI-HBP1; GPI-HBP1LOC338328; HBP1; High density lipoprotein-binding protein 1; HYPL1D

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