Recombinant Human GPR114 Protein, CF

Catalog # Availability Size / Price Qty
4747-GP-050
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Recombinant Human GPR114 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin. When 4 x 104 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL, Catalog # 1918-FN) and rhGPR114 (10 µg/well, 100 µL/well) for 60 minutes at 37° C, there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR114 protein
Glu22-Gly184, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu22
Predicted Molecular Mass
19.4 kDa
SDS-PAGE
40-55 kDa, reducing conditions

Product Datasheets

4747-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: GPR114

GPR114 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that exhibit long extracellular N-termini (1 - 3). The 528 amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion, followed by a GPCR proteolytic cleavage site (GPS) (2); it does not contain any other easily recognizable domains. Adhesion ligands have been reported for only a few LN-TM7 members (1). GPR114 is not one of them. Human GPR114 aa 22 - 184 share 63%, 63%, 67% and 59% aa identity with the corresponding regions of mouse, rat, bovine and equine GPR114, respectively. GPR114 was identified from expressed sequence tags (ESTs) found in leukemia cells, leukocytes, and pancreatic islets cells (2). An alternative start site has also been reported at Met 233 (4). One functional splice variant of GPR114 has been identified in colon, leukocytes, spleen and thymus (3).

References
  1. Bjarnadottir, T.K. et al. (2007) Cell. Mol. Life Sci. 64:2104.
  2. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  3. Bjarnadottir, T.K. et al. (2007) Gene 387:38.
  4. Accession # EAW82937.

Long Name
G Protein-coupled Receptor 114
Entrez Gene IDs
221188 (Human); 382045 (Mouse); 307645 (Rat)
Alternate Names
G protein-coupled receptor 114; G protein-coupled receptor PGR27; GPR114; G-protein coupled receptor PGR27; PGR27; PGR27G-protein coupled receptor 114; probable G-protein coupled receptor 114

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