Recombinant Human GPR114 Protein, CF

GPR114 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that exhibit long extracellular N-termini (1 - 3). The 528 amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion, followed by a GPCR proteolytic cleavage site (GPS) (2); it does not contain any other easily recognizable domains. Adhesion ligands have been reported for only a few LN-TM7 members (1). GPR114 is not one of them. Human GPR114 aa 22 - 184 share 63%, 63%, 67% and 59% aa identity with the corresponding regions of mouse, rat, bovine and equine GPR114, respectively. GPR114 was identified from expressed sequence tags (ESTs) found in leukemia cells, leukocytes, and pancreatic islets cells (2). An alternative start site has also been reported at Met 233 (4). One functional splice variant of GPR114 has been identified in colon, leukocytes, spleen and thymus (3).