Recombinant Human GPR114 Protein, CF
Recombinant Human GPR114 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Glu22-Gly184, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
GPR114 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that exhibit long extracellular N-termini (1 - 3). The 528 amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion, followed by a GPCR proteolytic cleavage site (GPS) (2); it does not contain any other easily recognizable domains. Adhesion ligands have been reported for only a few LN-TM7 members (1). GPR114 is not one of them. Human GPR114 aa 22 - 184 share 63%, 63%, 67% and 59% aa identity with the corresponding regions of mouse, rat, bovine and equine GPR114, respectively. GPR114 was identified from expressed sequence tags (ESTs) found in leukemia cells, leukocytes, and pancreatic islets cells (2). An alternative start site has also been reported at Met 233 (4). One functional splice variant of GPR114 has been identified in colon, leukocytes, spleen and thymus (3).
- Bjarnadottir, T.K. et al. (2007) Cell. Mol. Life Sci. 64:2104.
- Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
- Bjarnadottir, T.K. et al. (2007) Gene 387:38.
- Accession # EAW82937.
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