Recombinant Human GST-MDM2/HDM2 Protein, CF

Catalog # Availability Size / Price Qty
E3-202-050

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Recombinant Human GST-MDM2/HDM2 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human GST-Mdm2 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Mdm2 concentration of 0.5-4 μM.
Source
E. coli-derived human MDM2/HDM2 protein
Met1 - Pro491 with a N-terminal GST (glutathione S-transferase) tag
Accession #
Predicted Molecular Mass
82 kDa

Product Datasheets

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E3-202

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-202

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: MDM2/HDM2

Double minute 2 protein (Mdm2, also known as Hdm2) is a RING-finger Ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Mdm2 inhibits p53-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. The E3 ligase activity is confined to the C-terminal domain of Mdm2 and is responsible for the ubiquitination and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Mdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases autoubiquitination activity but increases activity toward p53. Mdm2 also affects the cell cycle and apoptosis through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5.

References
  1. Brooks C.L., et al. (2007) J.Biol.Chem 282:22804.
  2. Bushmann T., et al. (2001) J.Biol.Chem 276:40389.
  3. Dornan D, et al. (2004) Nature 429:86.
  4. Grossman S.R., et al. (2003) Science 300:342.
  5. Hu M., et al (2006) PLoS Biol. 4:228.
  6. Kawai H., et al. (2003) Mol. Cell. Biol. 23:4939.
  7. Lai Z., et al. (2001) J.Biol.Chem 276:31357.
  8. Li M, et al. (2003) Science 301:1972.
  9. Linares L.K., et al. (2003) Proc.Natl.Acad.Sci. 100:12009.
  10. Meulmeester E. and Jochemsen A.G. (2008) Curr.Canc.Drug. Targets 8:87.
  11. Sheng Y, et al. (2006) Nat.Struc.Mol.Biol. 13:285.
  12. Wu H., et al. (2011) Nature Med. 17:347.
  13. Yan Y. and Chen J. (2005) Biochem. Biophys. Res. Comm. 332:702.
  14. Yu G.W., et al. (2006) Proc.Natl.Acad.Sci. 103:1227.
Long Name
Double Minute 2 Protein
Entrez Gene IDs
4193 (Human); 17246 (Mouse)
Alternate Names
ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2

Citation for Recombinant Human GST-MDM2/HDM2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner
    Authors: JJ Wamsley, C Gary, A Biktasova, M Hajek, G Bellinger, R Virk, N Issaeva, WG Yarbrough
    Oncogenesis, 2017-04-10;6(4):e314.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay

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