Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10285-HE-050
Recombinant Human Hepassocin/FGL1 His-tag Protein, CF Binding Activity
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Recombinant Human Hepassocin/FGL1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 1 μg/mL (100 μL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05-0.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Hepassocin/FGL1 protein
Leu23-Ile312, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
35 kDa
SDS-PAGE
33-37 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10285-HE

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 1 μg/mL (100 μL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05-0.4 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) was resolved withSDS-PAGE under reducing (R) and non reducing (NR) conditions and visualized by Coomassie® Blue staining, showing single bands at 33-37 kDa and 65-75 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily, whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells, as it is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

References
  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.
Long Name
Fibrinogen Like 1
Entrez Gene IDs
2267 (Human); 246186 (Rat)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

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