Recombinant Human LAG-3 Fc Chimera Protein, CF

R&D Systems | Catalog # 2319-L3

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human LAG-3 Fc Chimera Protein (2319-L3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P18627

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all LAG-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAG-3 protein
Human LAG-3
(Leu23-Leu450)
Accession # P18627		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

72.7 kDa (monomer)

SDS-PAGE

75-90 kDa, under reducing conditions.

Activity

Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells.
The ED50 for this effect is 1-4 µg/mL.

Reviewed Applications

Read 4 reviews rated 5 using 2319-L3 in the following applications:

Formulation, Preparation, and Storage

2319-L3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.  Reconstitute 30 minutes prior to use with minimal agitation. 

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF) (1, 2). LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling (1, 2). Human LAG-3 cDNA encodes 525 amino acids (aa) that include a 28 aa signal sequence, a 422 aa extracellular domain (ECD) with four Ig-like domains, a  transmembrane region and a highly charged cytoplasmic region. Within the ECD, human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively. LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells (1-3). LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity (4). LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli (3-5). A soluble 54 kDa form, sLAG-3, can be shed by metalloproteinases ADAM10 and TACE/ADAM17 (6, 7). While monomeric sLAG-3 itself may be inactive, shedding allows for normal T cell activation by removing negative regulation (7). Binding of a homodimerized sLAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC, and secretion of cytokines such as IFN-gamma and TNF-alpha by type 1 cytotoxic CD8+ T cells and NK cells (8, 9). sLAG-3/Ig has been used as a potential adjuvant to stimulate a cytotoxic anti-cancer immune response (9, 10). In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases (11, 12). In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes (12-14).

References

  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  4. Huang, C.T. et al. (2004) Immunity 21:503.
  5. Workman, C.J. et al. (2009) J. Immunol. 182:1885.
  6. Li, N. et al. (2004) J. Immunol. 173:6806.
  7. Li, N. et al. (2007) EMBO J. 26:494.
  8. Andreae, S. et al. (2003) Blood 102:2130.
  9. Brignone, C. et al. (2007) J. Immunol. 179:4202.
  10. Brignone, C. et al. (2010) J. Transl. Med. 8:71.
  11. Woo, S.R. et al. (2011) Cancer Res. 72:917.
  12. Okazaki, T. et al. (2011) J. Exp. Med. 208:395.
  13. Bettini, M. et al. (2011) J. Immunol. 187:3493.
  14. Butler, N.S. et al. (2012) Nat. Immunol. 13:188.

Long Name

Lymphocyte-activation Gene 3

Alternate Names

CD223, LAG3

Entrez Gene IDs

3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)

Gene Symbol

LAG3

UniProt

P18627

Additional LAG-3 Products

Product Documents for Recombinant Human LAG-3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human LAG-3 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human LAG-3 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human LAG-3 Fc Chimera Protein, CF (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human LAG-3 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human LAG-3 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Proliferation
    Verified Customer | Posted 03/25/2019
  • Recombinant Human LAG-3 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Apoptosis assay
    Verified Customer | Posted 07/11/2018
  • Recombinant Human LAG-3 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/03/2017
  • Recombinant Human LAG-3 Fc Chimera Protein, CF
    Name: Anonymous
    Application: FACS sorting
    Verified Customer | Posted 06/16/2017
    FACS sorting of a yeast scFv library expressing LAG-3 binding antibodies.
    Yeast scFv that bind to LAG-3-Fc are in the upper right quadrant. The expected number of binders was observed and were later confirmed to be true LAG-3 binders.
    Recombinant Human LAG-3 Fc Chimera Protein, CF 2319-L3

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes