Prostaglandin D Synthase (PGDS) catalyzes the conversion of prostaglandin (PG) H2 to PGD2, which is a major prostanoid produced in a variety of tissues. Two types of PGDS have been isolated; the glutathione-dependent hematopoietic PGDS (HPGDS) and the glutathione-independent lipocalin‑type PGDS (1). HPGDS is a cytosolic enzyme that is expressed in mast cells and antigen presenting cells (2, 3). It is the only mammalian member of the class Sigma glutathione S‑transferase, showing a broad specificity towards standard transferase substrates (4). The PGD2 produced by HPGDS is involved in many physiological processes such as maintaining body temperature, promotion of sleep, inhibition of platelet aggregation and bronchoconstriction (5). It also functions in immune response and acts as a mediator in allergy and inflammation (6). HPGDS‑specific inhibitors may be therapeutically useful anti‑allergic and anti‑inflammatory drugs.
Recombinant Human HPGDS Protein, CF
R&D Systems | Catalog # 6487-PS
Discontinued Product
6487-PS has been discontinued.
View all Hematopoietic Prostaglandin D Synthase/HPGDS products.
Key Product Details
- R&D Systems E. coli-derived Recombinant Human HPGDS Protein (6487-PS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Hematopoietic Prostaglandin D Synthase/HPGDS protein
Met1-Leu199, with a C-terminal 6-His tag
Met1-Leu199, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro2
Predicted Molecular Mass
24 kDa
SDS-PAGE
24-26 kDa, reducing conditions
Activity
Measured by the conjugation of reduced glutathione to 1-bromo-2,4-dinitrobenzene.
The specific activity is >650 pmol/min/μg, as measured under the described conditions.
The specific activity is >650 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
6487-PS
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Hematopoietic Prostaglandin D Synthase/HPGDS
References
- Urade, Y. and Eguchi, N. (2002) Prostaglandins Other Lipid Mediat. 68:375.
- Urade, Y. et al. (1990) J. Biol. Chem. 265:371.
- Urade, Y. et al. (1989) J. Immunol. 143:2982.
- Jowsey, I. R. et al. (2001) Biochem. J. 359:507.
- Kanaoka, Y. and Urade, Y. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69:163.
- Oguma, T. et al. (2008) Allergol. Int. 57:307.
Alternate Names
GSTS, PGDS, PTGDS2
Gene Symbol
HPGDS
UniProt
Additional Hematopoietic Prostaglandin D Synthase/HPGDS Products
- All Products for Hematopoietic Prostaglandin D Synthase/HPGDS
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- Hematopoietic Prostaglandin D Synthase/HPGDS Lysates
- Hematopoietic Prostaglandin D Synthase/HPGDS Primary Antibodies
- Hematopoietic Prostaglandin D Synthase/HPGDS Proteins and Enzymes
Product Documents for Recombinant Human HPGDS Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human HPGDS Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human HPGDS Protein, CF (6487-PS):
Materials
*Adjusted for Substrate Blank
**Using the extinction coefficient 9600 M-1cm-1
***Using the path correction 0.320 cm
Note: the output of many spectrophotometers is in mOD. Per Well:
- Assay Buffer: 100 mM NaH2PO4, pH 7.0
- Recombinant Human Hematopoietic Prostaglandin D Synthase/HPGDS (rhHPGDS) (Catalog # 6487-PS)
- Substrate: 1-bromo-2,4-dinitrobenzene (BDNB) (Sigma, Catalog # 262226), 75 mM stock in ethanol
- L-Glutathione, reduced (GSH) (Amresco, Catalog # 399), 250 mM stock in deionized water
- UV Plate (Costar, Catalog # 3635)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhHPGDS to 40 ng/μL in Assay Buffer.
- Dilute the GSH to 4 mM in Assay Buffer.
- Combine equal volumes of 40 ng/μL rhHPGDS and 4 mM GSH for 20 ng/μL rhHPGDS with 2 mM GSH.
- Dilute Substrate to 2 mM in Assay Buffer.
- Load into a UV plate 50 μL of the 20 ng/μL rhHPGDS with 2 mM GSH mixture. Include a substrate blank containing 25 μL of Assay Buffer with 25 μL of the 4 mM GSH prepared in step 2.
- Start the reaction by adding 50 μL of 2 mM Substrate to wells.
- Read in kinetic mode for 5 minutes at an absorbance of 340 nm.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol |
| ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Using the extinction coefficient 9600 M-1cm-1
***Using the path correction 0.320 cm
Note: the output of many spectrophotometers is in mOD. Per Well:
- rhHPGDS: 1 μg
- GSH: 1 mM
- Substrate: 1 mM