Recombinant Human ICOS (aa 21-140) His-tag Protein, CF Summary
Accession # Q9Y6W8
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.5 μg/mL, Recombinant Human ICOS His-tag (Catalog # 9945-CS) binds withan ED50 of 0.7-3.5 ng/mL.
2 μg/lane of Recombinant Human ICOS was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 18-36 kDa and 40-72 kDa, respectively.
Inducible co-stimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors (1). Other family members are CD28, CTLA-4 and PD-1 (2, 4). Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (2, 4, 6). ICOS has been shown to have a role in the expansion of Th17 cells by regulating IL-21 production (8). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 6). The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Bauquet, AT. et al. (2009) Nat. Immunol. 10:167.
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