Recombinant Human ICOS (aa 21-140) His-tag Protein, CF Summary
Accession # Q9Y6W8
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.5 μg/mL, Recombinant Human ICOS His-tag (Catalog # 9945-CS) binds withan ED50 of 0.7-3.5 ng/mL.
2 μg/lane of Recombinant Human ICOS was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 18-36 kDa and 40-72 kDa, respectively.
Inducible co-stimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors (1). Other family members are CD28, CTLA-4 and PD-1 (2, 4). Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (2, 4, 6). ICOS has been shown to have a role in the expansion of Th17 cells by regulating IL-21 production (8). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 6). The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Bauquet, AT. et al. (2009) Nat. Immunol. 10:167.
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