Recombinant Human ICOS (aa 21-140) His-tag Protein, CF

Catalog # Availability Size / Price Qty
9945-CS-025
Recombinant Human ICOS (aa 21-140) His-tag Protein, CF Binding Activity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human ICOS (aa 21-140) His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human ICOS binds with an ED50 of 0.7-3.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ICOS protein
Human ICOS
(Glu21-Lys140)
Accession # Q9Y6W8
HHHHHH
IEGR
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu21
Structure / Form


Predicted Molecular Mass
15 kDa
SDS-PAGE
18-36 kDa, reducing conditions

Product Datasheets

9945-CS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human ICOS (aa 21-140) His-tag Protein, CF Binding Activity View Larger

When Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.5 μg/mL, Recombinant Human ICOS His-tag (Catalog # 9945-CS) binds withan ED50 of 0.7-3.5 ng/mL.

SDS-PAGE Recombinant Human ICOS (aa 21-140) His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human ICOS was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 18-36 kDa and 40-72 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors (1). Other family members are CD28, CTLA-4 and PD-1 (2, 4). Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (2, 4, 6). ICOS has been shown to have a role in the expansion of Th17 cells by regulating IL-21 production (8). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 6). The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).

References
  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  8. Bauquet, AT. et al. (2009) Nat. Immunol. 10:167.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

Reviews for Recombinant Human ICOS (aa 21-140) His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human ICOS (aa 21-140) His-tag Protein, CF and earn rewards!

Have you used Recombinant Human ICOS (aa 21-140) His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review