Recombinant Human IFN-beta Protein, CF

S.A approximately 2.8x10^8 IU/mg compared to WHO standard.
(2 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL.
    The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).Standard (NIBSC code: 00/572).

  • Source
    Chinese Hamster Ovary cell line, CHO-derived Met22-Asn187
  • Accession #
  • N-terminal Sequence
    Analysis
    Met22 & Ser23
  • Predicted Molecular Mass
    20 kDa
  • SDS-PAGE
    20-26 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8499-IF/CF
 
8499-IF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IFN-beta (Catalog # 8499-IF/CF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.
Background: IFN-beta
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
  • References:
    1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
    2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
    3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
    4. Häcker, H. et al. (2006) Nature 439:204.
    5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
    6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
    7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
    8. Matikainen, S. et al. (1999) Blood 93:1980.
    9. Teige, I. et al. (2003) J. Immunol. 170:4776.
    10. Shinohara, M.L. et al. (2008) Immunity 29:68.
    11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
    12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
    13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
    14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  • Long Name:
    Interferon beta
  • Entrez Gene IDs:
    3456 (Human); 15977 (Mouse); 24481 (Rat)
  • Alternate Names:
    Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
    Authors: HC Hsia, JE Hutti, AS Baldwin
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
    Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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