Recombinant Human IL-1 RAcP/IL-1 R3 Protein, CF Summary
Ser21-Glu359, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human IL-1 RAcP/IL-1 R3 (Catalog # 9176-CP) inhibits IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 0.8-4 μg/mL.
Background: IL-1 RAcP/IL-1 R3
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins (1). It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36 (2-4). It is a subunit of the functional signaling complex with IL-1 RI and associates with IL-1 RII in a non-signaling receptor complex (2, 5). In addition, it interacts with ST2/IL-1 R4 on mast cells and Th2 cells to create a functional IL-33 receptor complex (3). IL-1 RAcP also functions as a co-receptor for IL-36 alpha /IL-1F6, IL-36 beta /IL-1F8, and IL-36 gamma /IL-1F9 (4). Mature human IL-1 RAcP consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (2). Within the ECD, human IL-1 RAcP shares 86% aa sequence identity with mouse and rat IL-1 RAcP. Alternative splicing generates two secreted decoy receptor isoforms and an isoform with a substituted cytoplasmic domain (6-8). When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (9). Neuronal IL-1 RAcP interacts trans-synaptically with PTP sigma and can induce excitatory pre- and post-synaptic development (10).
- Boraschi, D. and A. Tagliabue (2013) Semin. Immunol. 25:394.
- Greenfeder, S.A. et al. (1995) J. Biol. Chem. 270:13757.
- Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
- Lang, D. et al. (1998) J. Immunol. 161:6871.
- Lu, H.-L. et al. (2008) Mol. Immunol. 45:1374.
- Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
- Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
- Smith, D.E. et al. (2003) Immunity 18:87.
- Yoshida, T. et al. (2012) J. Neurosci. 32:2588.
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