Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
872-RP-100
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhIL-1 F5, rhIL-1F8, rhIL-1F6 or rhIL-1F9 at 1 µg/mL (100 µL/well) can bind rhIL-1 Rrp2/Fc Chimera with a linear range of 0.15-5 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-1 Rrp2/IL-1 R6 protein
Human IL-1 R6
(Asp20 - Tyr337)
Accession # Q9HB29
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
62.8 kDa (monomer)
SDS-PAGE
80 kDa, reducing conditions

Product Datasheets

872-RP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-1 Rrp2/IL-1 R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1 Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a cofactor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

 

References
  1. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  2. Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
  3. Debets, R. et al. (2001) J. Immunol. 167:1440.
  4. Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 1 Receptor-like 2
Entrez Gene IDs
8808 (Human); 107527 (Mouse); 171106 (Rat)
Alternate Names
IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2

Citation for Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(8):3001-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Primary Antibodies

Human IgG1 Antibody

Human IgG1 Antibody

Recombinant Proteins

Reconstitution Buffers

Reviews for Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review