Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera Protein, CF

  (1 citations)     
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Citations (1)
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized rhIL-1 F5, rhIL-1F8, rhIL-1F6 or rhIL-1F9 at 1 µg/mL (100 µL/well) can bind rhIL-1 Rrp2/Fc Chimera with a linear range of 0.15-5 µg/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-1 Rrp2/IL-1 R6 protein
    Human IL-1 R6
    (Asp20 - Tyr337)
    Accession # Q9HB29
    DIEGRMD Human IgG1
    (Pro100 - Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp20
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    62.8 kDa (monomer)
  • SDS-PAGE
    80 kDa, reducing conditions
Product Datasheets

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872-RP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: IL-1 Rrp2/IL-1 R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1 Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a cofactor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

 

  • References:
    1. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    2. Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
    3. Debets, R. et al. (2001) J. Immunol. 167:1440.
    4. Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.
  • Long Name:
    Interleukin 1 Receptor-like 2
  • Entrez Gene IDs:
    8808 (Human); 107527 (Mouse); 171106 (Rat)
  • Alternate Names:
    IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(8):3001-5.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Binding Assay

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Primary Antibodies
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Human IgG1 Antibody

WB , ELISA MAB9894  
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Human IgG1 Antibody

WB MAB98941  
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Recombinant Proteins
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Recombinant Human IgG1 Fc Protein, CF

BA 110-HG 71
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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