Recombinant Human IL-11, ACFP Protein

Catalog # Availability Size / Price Qty
ACFP218
ACFP218-01M
R&D Systems Animal Component Free Recombinant Proteins
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Recombinant Human IL-11, ACFP Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
The specific activity of recombinant human IL-11 is approximately 1.05 x 104 units/μg, which is calibrated against recombinant human IL-11 WHO International Standard (NIBSC code: 92/788).
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human IL-11 protein
Pro22-Leu199
Produced in an animal component free process (ACFP).
Accession #
N-terminal Sequence
Analysis
Pro22
Predicted Molecular Mass
19 kDa
SDS-PAGE
20 kDa, reducing conditions

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ACFP218 (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ACFP218

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-3). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The human IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature human IL-11 shares 88%, 88%, and 96% aa sequence identity with mouse, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (4). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (5-7).

References
  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  5. Curtis, D.J. et al. (1997) Blood 90:4403.
  6. Baumann, H. et al. (1996) J. Immunol. 157:284.
  7. Karow, J. et al. (1996) Biochem. J. 318:489.
Long Name
Interleukin 11
Entrez Gene IDs
3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)
Alternate Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

Manufacturing Specifications

Animal Component-Free Process (ACFP) Manufacturing Conditions
R&D Systems Animal Component-Free Process (ACFP) recombinant proteins are expressed in an animal-free certified Sf 9 insect cell line using dedicated animal-free raw materials and labware. Production and purification procedures use equipment and media that are confirmed animal-free but performed outside our dedicated animal-free laboratories. Every stage of the manufacturing process follows R&D Systems' stringent Standard Operating Procedures (SOPs). The certified Sf 9 insect cell bank has undergone extensive testing to certify the lack of cytopathogens by screening for various viruses, Mycoplasma, and Spiroplasmas using both in vitro and in vivo testing methods. For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete ACFP Statement
 

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