Recombinant Human IL-12/IL-23 p40 Subunit Protein

R&D Systems | Catalog # 309-IL

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human IL-12/IL-23 p40 Subunit Protein (309-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P29460

Applications

Bioactivity
View all IL-12/IL-23 p40 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-12/IL-23 p40 protein
Ile23-Ser328

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

34.7 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL‑12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human IL‑12/IL‑23 p40 Subunit that produces 50% of the optimal binding response is 1-6 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 309-IL in the following applications:

Scientific Data Images for Recombinant Human IL-12/IL-23 p40 Subunit Protein

Recombinant Human IL-12/IL-23 p40 Monomer Protein Binding Activity

Recombinant Human IL-12/IL-23 p40 Subunit Protein Binding Activity

When Recombinant Human IL-12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Subunit (Catalog # 309-IL) binds with an ED50 of 1-6 ng/mL.

Formulation, Preparation, and Storage

309-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution

Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Long Name

Interleukin 12/Interleukin 23 p40

Alternate Names

CLMF2, IL-12B, IL12 p40, IL12B, NKSF2

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Gene Symbol

IL12B

UniProt

P29460

Additional IL-12/IL-23 p40 Products

Product Documents for Recombinant Human IL-12/IL-23 p40 Subunit Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-12/IL-23 p40 Subunit Protein

For research use only

Citations for Recombinant Human IL-12/IL-23 p40 Subunit Protein

Customer Reviews for Recombinant Human IL-12/IL-23 p40 Subunit Protein (1)

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  • Recombinant Human IL-12/IL-23 p40 Monomer Protein
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    Application: In vivo study
    Verified Customer | Posted 11/19/2024

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