Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

R&D Systems | Catalog # 839-B1

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IL-12 R beta 1 Fc Chimera Protein (839-B1)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P42701

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all IL-12 R beta 1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-12 R beta 1 protein
Human IL-12 R beta 1
(Cys24 - Glu540)
Accession # P42701		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

84.5 kDa (monomer)

SDS-PAGE

110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL-12 R beta 1 Fc Chimera (Catalog # 839-B1) binds Recombinant Human IL-12 (Catalog # 219-IL) with an ED50 of 1.40-14.0 ng/mL.

Reviewed Applications

Read 1 review rated 3 using 839-B1 in the following applications:

Formulation, Preparation, and Storage

839-B1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12 R beta 1

Interleukin 12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL-12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) residue protein with a putative 23 aa residue signal peptide that is cleaved to generate the mature protein with a 522 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 92 aa residue cytoplasmic region. Expression of IL-12 R beta 1 has been detected in activated T cells, NK cells and B cells. The expression of IL-12 R beta 2 is more restricted and appears to be limited to Th2 cells.

References

  1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

P42701

Additional IL-12 R beta 1 Products

Product Documents for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

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    Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF 839-B1

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