Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
839-B1-100

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Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhIL-12 R beta 1/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhIL-12 with a linear range of 0.78‑50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-12 R beta 1 protein
Human IL-12 R beta 1
(Cys24 - Glu540)
Accession # P42701
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Cys24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84.5 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions

Product Datasheets

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839-B1

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

839-B1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-12 R beta 1

Interleukin 12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL-12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) residue protein with a putative 23 aa residue signal peptide that is cleaved to generate the mature protein with a 522 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 92 aa residue cytoplasmic region. Expression of IL-12 R beta 1 has been detected in activated T cells, NK cells and B cells. The expression of IL-12 R beta 2 is more restricted and appears to be limited to Th2 cells.

References
  1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
Long Name
Interleukin 12 Receptor beta 1
Entrez Gene IDs
3594 (Human); 16161 (Mouse)
Alternate Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

Citations for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Preclinical development of a bispecific TNFalpha/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease
    Authors: KJ Roberts, MF Cubitt, TM Carlton, L Rodrigues-, L Maggiore, R Chai, S Clare, K Harcourt, TT MacDonald, KP Ray, A Vossenkämp, MR West, JS Crowe
    Scientific Reports, 2021;11(1):19422.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
    Authors: Yu RY, Gallagher G
    J. Immunol., 2010;185(12):7302-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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