Recombinant Human IL-12 Protein

(80 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.01-0.05 ng/mL.
    The specific activity of Recombinant Human IL-12 is approximately 1.1 x 104 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544).
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived
    Human IL-12 p40
    (Ile23-Ser328)
    Accession # P29460
    Human IL-12 p35
    (Arg23-Ser219)
    Accession # P29459
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ile23 (p40) & Arg23 (p35)
  • Structure / Form
    Disulfide-linked heterodimer
  • Predicted Molecular Mass
    34.7 kDa (p40) & 22.5 kDa (p35)
  • SDS-PAGE
    41 kDa (p40) & 29 kDa (p35), reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
219-IL
 
219-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-12 (Catalog #
219‑IL) stimulates proliferation in
PHA-activated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.
1 µg/lane of Recombinant Human IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. Single bands were observed at 41 kDa (p40) and 29 kDa (p35), under reducing conditions, and at 60 kDa under non-reducing conditions.
Background: IL-12
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

  • Long Name:
    Interleukin 12
  • Entrez Gene IDs:
    3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
  • Alternate Names:
    CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

80 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. EBI3 regulates the NK cell response to mouse cytomegalovirus infection
    Authors: H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Retinoic-acid-orphan-receptor-C inhibition suppresses Th17 cells and induces thymic aberrations
    Authors: C Guntermann, A Piaia, ML Hamel, D Theil, T Rubic-Schn, A Del Rio-Es, L Dong, A Billich, K Kaupmann, J Dawson, K Hoegenauer, D Orain, S Hintermann, R Stringer, DD Patel, A Doelemeyer, M Deurinck, J Schümann
    JCI Insight, 2017;2(5):e91127.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
    Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
    JCI Insight, 2017;2(9):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017;18(1):159.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Tonsillar CD56brightNKG2A+ NK cells restrict primary Epstein-Barr virus infection in B cells via IFN-?
    Authors: A Jud, M Kotur, C Berger, C Gysin, D Nadal, A Lünemann
    Oncotarget, 2017;8(4):6130-6141.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression
    Authors: H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Interferon Alpha Induces Sustained Changes in NK Cell Responsiveness to Hepatitis B Viral Load Suppression In Vivo
    PLoS Pathog, 2016;12(8):e1005788.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture
    Authors: Burak Dura
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Preclinical validation: LV/IL-12 transduction of patient leukemia cells for immunotherapy of AML
    Mol Ther Methods Clin Dev, 2016;3(0):16074.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Ctrl
  11. Innate lymphoid cells are expanded and functionally altered in chronic lymphocytic leukemia
    Authors: I de Weerdt, V van Hoeven, JM Munneke, S Endstra, T Hofland, MD Hazenberg, AP Kater
    Haematologica, 2016;101(11):e461-e464.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Survivin-specific T cell receptor targets tumor but not T cells.
    Authors: Arber C, Feng X, Abhyankar H, Romero E, Wu M, Heslop H, Barth P, Dotti G, Savoldo B
    J Clin Invest, 2015;125(1):157-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin.
    Authors: Kudo H, Wang Z, Jinnin M, Nakayama W, Inoue K, Honda N, Nakashima T, Kajihara I, Makino K, Makino T, Fukushima S, Ihn H
    J Immunol, 2015;195(8):3565-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. MicroRNA-146a controls Th1-cell differentiation of human CD4+ T lymphocytes by targeting PRKCepsilon.
    Authors: Mohnle P, Schutz S, van der Heide V, Hubner M, Luchting B, Sedlbauer J, Limbeck E, Hinske L, Briegel J, Kreth S
    Eur J Immunol, 2015;45(1):260-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015;56(2):1051-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Broadly-specific cytotoxic T cells targeting multiple HIV antigens are expanded from HIV+ patients: implications for immunotherapy.
    Authors: Lam, Sharon, Sung, Julia, Cruz, Conrad, Castillo-Caro, Paul, Ngo, Minhtran, Garrido, Carolina, Kuruc, Joann, Archin, Nancie, Rooney, Cliona, Margolis, David, Bollard, Catherin
    Mol Ther, 2015;23(2):387-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. GIMAP GTPase family genes: potential modifiers in autoimmune diabetes, asthma, and allergy.
    Authors: Heinonen M, Laine A, Soderhall C, Gruzieva O, Rautio S, Melen E, Pershagen G, Lahdesmaki H, Knip M, Ilonen J, Henttinen T, Kere J, Lahesmaa R
    J Immunol, 2015;194(12):5885-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Evidence for eomesodermin-expressing innate-like CD8(+) KIR/NKG2A(+) T cells in human adults and cord blood samples.
    Authors: Jacomet F, Cayssials E, Basbous S, Levescot A, Piccirilli N, Desmier D, Robin A, Barra A, Giraud C, Guilhot F, Roy L, Herbelin A, Gombert J
    Eur J Immunol, 2015;45(7):1926-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. STAT3 is a critical cell-intrinsic regulator of human unconventional T cell numbers and function.
    Authors: Wilson R, Ives M, Rao G, Lau A, Payne K, Kobayashi M, Arkwright P, Peake J, Wong M, Adelstein S, Smart J, French M, Fulcher D, Picard C, Bustamante J, Boisson-Dupuis S, Gray P, Stepensky P, Warnatz K, Freeman A, Rossjohn J, McCluskey J, Holland S, Casanova J, Uzel G, Ma C, Tangye S, Deenick E
    J Exp Med, 2015;212(6):855-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Identification of Four-Jointed Box 1 (FJX1)-Specific Peptides for Immunotherapy of Nasopharyngeal Carcinoma.
    Authors: Chai S, Yap Y, Foo Y, Yap L, Ponniah S, Teo S, Cheong S, Patel V, Lim K
    PLoS ONE, 2015;10(11):e0130464.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
    Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
    Nat Immunol, 2015;16(3):318-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Manipulating the expression of chemokine receptors enhances delivery and activity of cytokine-induced killer cells.
    Authors: Zou Y, Li F, Hou W, Sampath P, Zhang Y, Thorne S
    Br J Cancer, 2014;110(8):1992-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Flow cytometric detection of p38 MAPK phosphorylation and intracellular cytokine expression in peripheral blood subpopulations from patients with autoimmune rheumatic diseases.
    Authors: Mavropoulos A, Bogdanos D, Liaskos C, Orfanidou T, Simopoulou T, Zafiriou E, Sakkas L, Rigopoulou E
    J Immunol Res, 2014;2014(0):671431.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. The regulation and role of c-FLIP in human Th cell differentiation.
    Authors: Kylaniemi M, Kaukonen R, Myllyviita J, Rasool O, Lahesmaa R
    PLoS ONE, 2014;9(7):e102022.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Combinatorial flexibility of cytokine function during human T helper cell differentiation.
    Authors: Touzot M, Grandclaudon M, Cappuccio A, Satoh T, Martinez-Cingolani C, Servant N, Manel N, Soumelis V
    Nat Commun, 2014;5(0):3987.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Decreased RORC-dependent silencing of prostaglandin receptor EP2 induces autoimmune Th17 cells.
    Authors: Kofler D, Marson A, Dominguez-Villar M, Xiao S, Kuchroo V, Hafler D
    J Clin Invest, 2014;124(6):2513-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
    Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
    PLoS ONE, 2014;9(5):e96695.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
    Authors: Duhen T, Campbell D
    J Immunol, 2014;193(1):120-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Mapping the p53 transcriptome universe using p53 natural polymorphs.
    Authors: Wang, B, Niu, D, Lam, T H, Xiao, Z, Ren, E C
    Cell Death Differ, 2014;21(4):521-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of Interleukin-1 receptor antagonist.
    Authors: Gresnigt M, Bozza S, Becker K, Joosten L, Abdollahi-Roodsaz S, van der Berg W, Dinarello C, Netea M, Fontaine T, De Luca A, Moretti S, Romani L, Latge J, van de Veerdonk F
    PLoS Pathog, 2014;10(3):e1003936.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. TL1A induces TCR independent IL-6 and TNF-alpha production and growth of PLZF(+) leukocytes.
    Authors: Reichwald K, Jorgensen T, Tougaard P, Skov S
    PLoS ONE, 2014;9(1):e85793.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. High activities of BACE1 in brains with mild cognitive impairment.
    Authors: Cheng X, He P, Lee T, Yao H, Li R, Shen Y
    Am J Pathol, 2014;184(1):141-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Global chromatin state analysis reveals lineage-specific enhancers during the initiation of human T helper 1 and T helper 2 cell polarization.
    Authors: Hawkins R, Larjo A, Tripathi S, Wagner U, Luu Y, Lonnberg T, Raghav S, Lee L, Lund R, Ren B, Lahdesmaki H, Lahesmaa R
    Immunity, 2013;38(6):1271-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Kynurenine 3-monooxygenase mediates inhibition of Th17 differentiation via catabolism of endogenous aryl hydrocarbon receptor ligands.
    Authors: Stephens G, Wang Q, Swerdlow B, Bhat G, Kolbeck R, Fung M
    Eur J Immunol, 2013;43(7):1727-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  37. Adiponectin modulates NK-cell function.
    Authors: Wilk S, Jenke A, Stehr J, Yang C, Bauer S, Goldner K, Kotsch K, Volk H, Poller W, Schultheiss H, Skurk C, Scheibenbogen C
    Eur J Immunol, 2013;43(4):1024-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  38. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
    Authors: Chen, Qian, Swaminathan, Sanjay, Yang, De, Dai, Lue, Sui, Hongyan, Yang, Jun, Hornung, Ronald L, Wang, Yanmei, Huang, Da Wei, Hu, Xiaojun, Lempicki, Richard, Imamichi, Tomozumi
    PLoS ONE, 2013;8(3):e59194.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. Neutrophil microparticles modulate cytokine production by natural killer cells.
    Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
    Cytokine, 2013;65(2):126-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  41. Loss of methylation at the IFNG promoter and CNS-1 is associated with the development of functional IFN-gamma memory in human CD4(+) T lymphocytes.
    Authors: Dong J, Chang H, Ivascu C, Qian Y, Rezai S, Okhrimenko A, Cosmi L, Maggi L, Eckhardt F, Wu P, Sieper J, Alexander T, Annunziato F, Gossen M, Li J, Radbruch A, Thiel A
    Eur J Immunol, 2013;43(3):793-804.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS.
    Authors: Flanagan K, Fitzgerald K, Baker J
    PLoS ONE, 2012;7(7):e40443.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. AAV2/IL-12 gene delivery into dendritic cells (DC) enhances CTL stimulation above other IL-12 applications: Evidence for IL-12 intracrine activity in DC.
    Authors: You C, Shi M, Liu Y, Cao M, Luo R, Hermonat P
    Oncoimmunology, 2012;1(6):847-855.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Inter-regulation of Th17 cytokines and the IL-36 cytokines in vitro and in vivo: implications in psoriasis pathogenesis.
    Authors: Carrier Y, Ma HL, Ramon HE, Napierata L, Small C, O'Toole M, Young DA, Fouser LA, Nickerson-Nutter C, Collins M, Dunussi-Joannopoulos K, Medley QG
    J. Invest. Dermatol., 2011;131(12):2428-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies.
    Authors: Le Garff-Tavernier M, Decocq J, de Romeuf C, Parizot C, Dutertre CA, Chapiro E, Davi F, Debre P, Prost JF, Teillaud JL, Merle-Beral H, Vieillard V
    Leukemia, 2011;25(1):101-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  48. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2010;184(3):1642-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  49. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010;5(8):e11966.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. Natural killer cell-mediated cytokine response among HIV-positive south Indians with pulmonary tuberculosis.
    Authors: Ramana Rao PV, Rajasekaran S, Raja A
    J. Interferon Cytokine Res., 2010;30(1):33-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
    Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
    Eur. J. Immunol., 2010;40(9):2450-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
    Authors: Jindra C
    Eur J Immunol, 2010;40(2):321-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009;183(9):5494-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  54. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  55. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009;10(8):702-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  56. Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
    Authors: De Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
    Arthritis Rheum., 2009;60(9):2782-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  57. Fully functional NK cells after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Lapusan S, Hervier B, Dhedin N, Bories D, Uzunov M, Boudifa A, Trebeden-Negre H, Norol F, Marjanovic Z, Marie JP, Vernant JP, Debre P, Rio B, Vieillard V
    Leukemia, 2009;23(4):721-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease.
    Authors: Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL
    J. Clin. Immunol., 2009;29(3):319-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  59. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  60. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  61. Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion.
    Authors: de Matos CT, Berg L, Michaelsson J, Fellander-Tsai L, Karre K, Soderstrom K
    Immunology, 2007;122(2):291-301.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  63. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  64. IL-24 modulates IFN-gamma expression in patients with tuberculosis.
    Authors: Wu B, Huang C, Kato-Maeda M, Hopewell PC, Daley CL, Krensky AM, Clayberger C
    Immunol. Lett., 2007;117(1):57-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  65. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
    Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
    Blood, 2006;107(9):3617-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  67. Human complete Stat-1 deficiency is associated with defective type I and II IFN responses in vitro but immunity to some low virulence viruses in vivo.
    Authors: Chapgier A, Wynn RF, Jouanguy E, Filipe-Santos O, Zhang S, Feinberg J, Hawkins K, Casanova JL, Arkwright PD
    J. Immunol., 2006;176(8):5078-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
    Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
    J. Immunol., 2006;176(12):7768-74.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Specificity Testing
  69. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  70. CYP7B expression and activity in fibroblast-like synoviocytes from patients with rheumatoid arthritis: regulation by proinflammatory cytokines.
    Authors: Dulos J, van der Vleuten MA, Kavelaars A, Heijnen CJ, Boots AM
    Arthritis Rheum., 2005;52(3):770-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  71. Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma.
    Authors: Radstake TR, Roelofs MF, Jenniskens YM, van Riel PL, Barrera P
    Arthritis Rheum., 2004;50(12):3856-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004;104(7):2095-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  73. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  74. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  75. APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
    Authors: Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
    J. Immunol., 2004;172(1):138-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.
    Authors: Moreaux J, Legouffe E, Jourdan E, Quittet P, Reme T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
    Blood, 2004;103(8):3148-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
    Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
    Blood, 2003;102(12):4084-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Cutting edge: immune cells as sources and targets of the IL-10 family members?
    Authors: Wolk K, Kunz S, Asadullah K, Sabat R
    J. Immunol., 2002;168(11):5397-402.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. Lack of an immune response against the tetracycline-dependent transactivator correlates with long-term doxycycline-regulated transgene expression in nonhuman primates after intramuscular injection of recombinant adeno-associated virus.
    Authors: Favre D, Blouin V, Provost N, Spisek R, Porrot F, Bohl D, Marme F, Cherel Y, Salvetti A, Hurtrel B, Heard JM, Riviere Y, Moullier P
    J. Virol., 2002;76(22):11605-11.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  80. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R.
    Authors: Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O&amp;amp;apos;Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW
    J. Immunol., 2002;168(11):5699-708.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 80 Citations

Have you used Recombinant Human IL-12 Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information