Recombinant Human IL-12 R beta 1 His-tag Protein, CF

Name: Recombinant Human IL-12 R beta 1 His-tag Protein, CF
Brand: R&D Systems
SKU: 11310-B1
Price: 445 USD
Availability: InStock

R&D Systems | Catalog # 11310-B1

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human IL-12 R beta 1 His-tag Protein (11310-B1)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P42701.1

Applications

Bioactivity

View all IL-12 R beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 1 protein
Cys24-Glu540, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

58 kDa

SDS-PAGE

62-79 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL‑12 R beta 1 His-tag binds to Recombinant Human IL‑12 (Catalog # 219-IL) with an ED₅₀ of 3.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human IL-12 R beta 1 His-tag Protein, CF

Recombinant Human IL‑12 R beta 1 His-tag Protein SEC-MALS.

Recombinant Human IL‑12 R beta 1 His-tag Protein (Catalog # 11310-B1) has a molecular weight (MW) of 71.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human IL‑12 R beta 1 His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human IL‑12 R beta 1 His-tag (Catalog # 11310-B1) binds to Recombinant Human IL‑12 (219-IL) with an ED₅₀ of 3.00-30.0 ng/mL.

Recombinant Human IL-12 R beta 1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-12 R beta 1 His-tag Protein (Catalog # 11310-B1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-79 kDa.

Formulation, Preparation, and Storage

11310-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-12 R beta 1

IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).