Recombinant Human IL-17A/F Heterodimer Protein

Newer Version Available: 5837-IL
NEW
A New rh IL-17A/F is Now Available! It has ~10 fold better activity and is CHO expressed!

Carrier Free

Catalog # Availability Size / Price Qty
5194-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5194-IL-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
FAQs
Reviews (3)

Recombinant Human IL-17A/F Heterodimer Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived human IL-17A/F Heterodimer protein
MetHuman IL-17A
(Ile20-Ala155)
Accession # Q16552
MetHuman IL-17F
(Arg31-Gln163)
Accession # Q96PD4
N-terminusC-terminus
N-terminal Sequence
Analysis
Met (IL-17A) & Met (IL-17F)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
IL-17A: 15.7 kDa and IL-17F: 15 kDa

Product Datasheets

5194-IL (with carrier)

5194-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5194-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5194-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17A/F Heterodimer

Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References
  1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  5. Gu, C. et al. (2013) Cytokine 64:477.
  6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  8. Ouyang, W. et al. (2008) Immunity 28:454.
  9. Steinman, L. (2007) Nat. Med. 13:139.
  10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
Long Name
Interleukin 17 A/F
Alternate Names
CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A

Citations for Recombinant Human IL-17A/F Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015;212(5):619-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
    Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
    J. Exp. Med., 2010;207(2):299-308.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)

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Reviews for Recombinant Human IL-17A/F Heterodimer Protein

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Recombinant Human IL-17A/F Heterodimer Protein
By Anonymous on 10/28/2020
Application: Media additive for protein or antibody production

Recombinant Human IL-17A/F Heterodimer Protein
By Anonymous on 11/22/2019
Application: Media additive for protein or antibody production

Recombinant Human IL-17A/F Heterodimer Protein
By Anonymous on 01/09/2018
Application: Immunoassay Standard