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Recombinant Human IL-17A Protein, CF

R&D Systems | Catalog # 317-ILB

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-17A Protein (317-ILB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q16552

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-17/IL-17A Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-17/IL-17A protein
Ile20-Ala155, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa (monomer)

Activity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells.
The ED50 for this effect is 0.4-4 ng/mL.

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is 1.5-7.5 ng/mL.

Reviewed Applications

Read 5 reviews rated 4.8 using 317-ILB in the following applications:

Scientific Data Images for Recombinant Human IL-17A Protein, CF

Recombinant Human IL-17A Protein Bioactivity

Recombinant Human IL-17A Protein Bioactivity

Recombinant Human IL-17 (Catalog # 317-ILB) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.4-4 ng/mL.

Formulation, Preparation, and Storage

317-ILB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100-500 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma / delta T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

References

  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  17. Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for Recombinant Human IL-17A Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-17A Protein, CF

For research use only

Citations for Recombinant Human IL-17A Protein, CF

Customer Reviews for Recombinant Human IL-17A Protein, CF (5)

4.8 out of 5
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  • Recombinant Human IL-17A Protein, CF
    Name: Anonymous
    Application: THP-1 cells
    Verified Customer | Posted 05/12/2025
    THP-1 stimulated
    Recombinant Human IL-17A Protein, CF 317-ILB
  • Recombinant Human IL-17A Protein, CF
    Name: Zhenping Wang
    Application: Fibroblasts migration
    Verified Customer | Posted 09/30/2020
    Used for fibroblasts migration in a modified Boyden chamber with a membrane pore size of 8 μm.
    Recombinant Human IL-17A Protein, CF 317-ILB
  • Recombinant Human IL-17A Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 08/22/2018
    Recombinant Human IL-17A Protein, CF 317-ILB
  • Recombinant Human IL-17A Protein, CF
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 01/18/2018
  • Recombinant Human IL-17A Protein, CF
    Name: Anonymous
    Application: Characterization prior to use.
    Verified Customer | Posted 01/05/2018
    Recombinant Human IL-17A Protein, CF 317-ILB

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FAQs for Recombinant Human IL-17A Protein, CF

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  • Q: What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?

    A: There are multiple differences between Catalog # 317-ILB and 7955-IL.

    • 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells.
    • Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein).
    • Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

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