Recombinant Human IL-17B (CHO) Protein

  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. Tang, Y. et al. (2011) Clin. Exp. Immunol. 166:281. The ED50 for this effect is 0.4-2 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Gln21-Phe180
  • Accession #
  • N-terminal Sequence
    Analysis
    Gln21, Ser51 & Gly102
    Gln21 predicted, sequencing might be blocked
  • Structure / Form
    Noncovalently-linked homodimer
  • Predicted Molecular Mass
    18 kDa (monomer)
  • SDS-PAGE
    20-25 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8129-IL
 
8129-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
IL-17B-induced IL-8 Secretion.
Recombinant Human IL-17B induces IL-8 secretion by HepG2 human hepatocellular carcinoma cells.
Background: IL-17B
Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively (3, 4). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17 RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10).
  • References:
    1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
    2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
    3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
    4. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
    5. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
    6. Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
    7. You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
    8. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
    9. Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
    10. Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.
  • Long Name:
    Interleukin 17B
  • Entrez Gene IDs:
    27190 (Human); 56069 (Mouse)
  • Alternate Names:
    Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20
Related Research Areas

Have you used Recombinant Human IL-17B (CHO) Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information