Recombinant Human IL-18/IL-1F4 Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 1.5-9 ng/mL.
  • Source
    E. coli-derived Tyr37-Asp193
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr37
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    19 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9124-IL/CF
 
9124-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-18 (Catalog #
9124-IL/CF) induces IFN-gamma secretion by
KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5-9 ng/mL.
1 μg/lane of Recombinant Human
IL-18/IL-1F4 (Catalog # 9124-IL/CF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.
Background: IL-18/IL-1F4
Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).
  • References:
    1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
    2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
    3. Gu, Y. et al. (1997) Science 275:206.
    4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
    5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
    6. Novick, D. et al. (1999) Immunity 10:127.
    7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
    8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
    9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
    10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
    11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
    12. Li, J. et al. (2004) Cell. Immunol. 227:103.
    13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
    14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
    15. Ushio, S. et al. (1996) J. Immunol. 156:4274.
    16. Gaggero, A. et al. (2004) Oncogene 23:7552.
    17. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.
  • Long Name:
    Interleukin 18
  • Entrez Gene IDs:
    3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
  • Alternate Names:
    Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. EBI3 regulates the NK cell response to mouse cytomegalovirus infection
    Authors: H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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