hh Recombinant Human IL-3 Protein 203-IL-010: R&D Systems

Recombinant Human IL-3 Protein

(100 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL.
    The specific activity of Recombinant Human IL-3 is approximately 1.8 x 103 IU/μg, which is calibrated against recombinant human IL-3 WHO International Standard (NIBSC code: 91/510).
  • Source
    E. coli-derived Ala20-Phe152, with and without an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala20 and Met
  • Predicted Molecular Mass
    15 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
203-IL
 
203-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.1 ng/mL.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

  • Long Name:
    Interleukin 3
  • Entrez Gene IDs:
    3562 (Human); 16187 (Mouse); 24495 (Rat)
  • Alternate Names:
    Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

100 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)
    Authors: E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés
    Stem Cell Res, 2017;21(0):19-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Whole-Genome Sequencing of a Family with Hereditary Pulmonary Alveolar Proteinosis Identifies a Rare Structural Variant Involving CSF2RA/CRLF2/IL3RA Gene Disruption
    Authors: CY Chiu, SC Su, WL Fan, SH Lai, MH Tsai, SH Chen, KS Wong, WH Chung
    Sci Rep, 2017;7(0):43469.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
    Authors: AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells
    Authors: K Trakarnsan, RE Griffiths, MC Wilson, A Blair, TJ Satchwell, M Meinders, N Cogan, S Kupzig, R Kurita, Y Nakamura, AM Toye, DJ Anstee, J Frayne
    Nat Commun, 2017;8(0):14750.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Human Plasmacytoid Dendritic Cells Display and Shed B Cell Maturation Antigen upon TLR Engagement
    Authors: E Schuh, A Musumeci, FS Thaler, S Laurent, JW Ellwart, R Hohlfeld, A Krug, E Meinl
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
    Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis
    Authors: L Butcher, M Ahluwalia, T Örd, J Johnston, RH Morris, E Kiss-Toth, T Örd, JD Erusalimsk
    Sci Rep, 2017;7(1):6684.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis
    Authors: NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry
    Cell Rep, 2017;20(7):1597-1608.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise
    Authors: H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease
    Authors: J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin
    Mol Ther Methods Clin Dev, 2017;5(0):241-258.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC Induced Thrombocytopenia
    Authors: H Zhao, S Gulesseria, SK Ganesan, J Ou, K Morrison, Z Zeng, V Robles, J Snyder, L Do, H Aviña, S Karki, DR Stover, F Doñate
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
    Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
    Nat Commun, 2017;8(0):15380.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
    Cell Biosci, 2016;6(1):50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Preclinical validation: LV/IL-12 transduction of patient leukemia cells for immunotherapy of AML
    Mol Ther Methods Clin Dev, 2016;3(0):16074.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Ras oncogene-independent activation of RALB signaling is a targetable mechanism of escape from NRAS(V12) oncogene addiction in acute myeloid leukemia
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
    Blood, 2016;127(23):2879-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Leucine-Rich Repeat Kinase 2 Influences Fate Decision of Human Monocytes Differentiated from Induced Pluripotent Stem Cells
    PLoS ONE, 2016;11(11):e0165949.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors
    Sci Rep, 2016;6(0):32149.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. A New Glucocerebrosidase Chaperone Reduces ?-Synuclein and Glycolipid Levels in iPSC-Derived Dopaminergic Neurons from Patients with Gaucher Disease and Parkinsonism
    J Neurosci, 2016;36(28):7441-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24).
    Authors: Dyer, Kimberly, Rosenberg, Helene F
    PLoS ONE, 2015;10(3):e0118887.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
    Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
    Stem Cells, 2015;33(5):1554-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues.
    Authors: Patterson, Scott D, Rossi, John M, Paweletz, Katherin, Fitzpatrick, V Dan, Begley, C Glenn, Busse, Leigh, Elliott, Steve, McCaffery, Ian
    PLoS ONE, 2015;10(3):e0122149.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Increased IFN-alpha-producing plasmacytoid dendritic cells (pDCs) in human Th1-mediated type 1 diabetes: pDCs augment Th1 responses through IFN-alpha production.
    Authors: Xia C, Peng R, Chernatynskaya A, Yuan L, Carter C, Valentine J, Sobel E, Atkinson M, Clare-Salzler M
    J Immunol, 2015;193(3):1024-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  27. Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
    Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
    Mol Ther, 2015;23(2):339-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia.
    Authors: Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M
    Leukemia, 2015;29(12):2382-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Preserved in vivo reconstitution ability of PBSCs cryopreserved for a decade at -80 degrees C.
    Authors: Shima T, Iwasaki H, Yamauchi T, Kadowaki M, Kiyosuke M, Mochimaru T, Takenaka K, Miyamoto T, Akashi K, Teshima T
    Bone Marrow Transplant, 2015;50(9):1195-200.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Suppression of basophil FcvarepsilonRI activation by serum from active chronic idiopathic/spontaneous urticaria (CIU/CSU) subjects.
    Authors: Sterba P, Hamilton R, Saini S
    J Invest Dermatol, 2015;135(5):1454-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
    Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
    PLoS ONE, 2015;10(4):e0122983.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015;33(4):1142-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells.
    Authors: Purushothaman P, Thakker S, Verma S
    J Virol, 2015;89(6):3093-111.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. Enrichment of human hematopoietic stem/progenitor cells facilitates transduction for stem cell gene therapy.
    Authors: Baldwin K, Urbinati F, Romero Z, Campo-Fernandez B, Kaufman M, Cooper A, Masiuk K, Hollis R, Kohn D
    Stem Cells, 2015;33(5):1532-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Protection of human myeloid dendritic cell subsets against influenza A virus infection is differentially regulated upon TLR stimulation.
    Authors: Baharom F, Thomas S, Bieder A, Hellmer M, Volz J, Sandgren K, McInerney G, Karlsson Hedestam G, Mellman I, Smed-Sorensen A
    J Immunol, 2015;194(9):4422-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells.
    Authors: Jensen B, Frandsen P, Raaby E, Schiotz P, Skov P, Poulsen L
    J Leukoc Biol, 2014;95(6):893-901.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. MUC1-C oncoprotein promotes FLT3 receptor activation in acute myeloid leukemia cells.
    Authors: Liu, Suiyang, Yin, Li, Stroopinsky, Dina, Rajabi, Hasan, Puissant, Alexandr, Stegmaier, Kimberly, Avigan, David, Kharbanda, Surender, Kufe, Donald, Stone, Richard
    Blood, 2014;123(5):734-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
    Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
    Blood, 2014;123(8):1239-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Neutralizing antibodies inhibit HIV-1 infection of plasmacytoid dendritic cells by an FcgammaRIIa independent mechanism and do not diminish cytokines production.
    Authors: Lederle A, Su B, Holl V, Penichon J, Schmidt S, Decoville T, Laumond G, Moog C
    Sci Rep, 2014;4(0):5845.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. Human induced pluripotent stem cell derived erythroblasts can undergo definitive erythropoiesis and co-express gamma and beta globins.
    Authors: Yang C, French A, Goh P, Pagnamenta A, Mettananda S, Taylor J, Knight S, Nathwani A, Roberts D, Watt S, Carpenter L
    Br J Haematol, 2014;166(3):435-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  41. A novel formulation of tigecycline has enhanced stability and sustained antibacterial and antileukemic activity.
    Authors: Jitkova, Yulia, Gronda, Marcela, Hurren, Rose, Wang, Xiaoming, Goard, Carolyn, Jhas, Bozhena, Schimmer, Aaron D
    PLoS ONE, 2014;9(5):e95281.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. CCK8 negatively regulates the TLR9-induced activation of human peripheral blood pDCs by targeting TRAF6 signaling.
    Authors: Jia X, Cong B, Zhang J, Li H, Liu W, Chang H, Dong M, Ma C
    Eur J Immunol, 2014;44(2):489-99.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. Stress hematopoiesis is regulated by the Kruppel-like transcription factor ZBP-89.
    Authors: Li X, Romain R, Park D, Scadden D, Merchant J, Arnaout M
    Stem Cells, 2014;32(3):791-801.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. Gene transfer properties and structural modeling of human stem cell-derived AAV.
    Authors: Smith, Laura J, Ul-Hasan, Taihra, Carvaines, Sarah K, Van Vliet, Kim, Yang, Ethel, Wong, Kamehame, Agbandje-McKenna, Mavis, Chatterjee, Saswati
    Mol Ther, 2014;22(9):1625-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. Blockade of type I interferon (IFN) production by retroviral replicating vectors and reduced tumor cell responses to IFN likely contribute to tumor selectivity.
    Authors: Lin A, Burrascano C, Pettersson P, Ibanez C, Gruber H, Jolly D
    J Virol, 2014;88(17):10066-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis.
    Authors: Morishima, Tatsuya, Watanabe, Ken-ichi, Niwa, Akira, Hirai, Hideyo, Saida, Satoshi, Tanaka, Takayuki, Kato, Itaru, Umeda, Katsutsu, Hiramatsu, Hidefumi, Saito, Megumu K, Matsubara, Kousaku, Adachi, Souichi, Kobayashi, Masao, Nakahata, Tatsutos, Heike, Toshio
    Haematologica, 2014;99(1):19-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells.
    Authors: Kamat V, Paluru P, Myint M, French D, Gadue P, Diamond S
    Stem Cells, 2014;32(5):1337-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  48. The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.
    Authors: van Wilgenburg, Bonnie, Moore, Michael, James, William, Cowley, Sally A
    PLoS ONE, 2014;9(1):e86071.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  49. Epigenetic reprogramming induces the expansion of cord blood stem cells.
    Authors: Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R
    J Clin Invest, 2014;124(6):2378-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
    Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
    J Invest Dermatol, 2014;134(5):1246-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells.
    Authors: Takahashi, M, Matsuoka, Y, Sumide, K, Nakatsuka, R, Fujioka, T, Kohno, H, Sasaki, Y, Matsui, K, Asano, H, Kaneko, K, Sonoda, Y
    Leukemia, 2014;28(6):1308-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. Attenuation of pathogenic immune responses during infection with human and simian immunodeficiency virus (HIV/SIV) by the tetracycline derivative minocycline.
    Authors: Drewes J, Szeto G, Engle E, Liao Z, Shearer G, Zink M, Graham D
    PLoS ONE, 2014;9(4):e94375.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. NADPH oxidase-independent formation of extracellular DNA traps by basophils.
    Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
    J Immunol, 2014;192(11):5314-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  54. Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.
    Authors: Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C
    Blood, 2014;123(20):3116-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  55. Allelic exclusion and peripheral reconstitution by TCR transgenic T cells arising from transduced human hematopoietic stem/progenitor cells.
    Authors: Giannoni F, Hardee C, Wherley J, Gschweng E, Senadheera S, Kaufman M, Chan R, Bahner I, Gersuk V, Wang X, Gjertson D, Baltimore D, Witte O, Economou J, Ribas A, Kohn D
    Mol Ther, 2013;21(5):1044-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  56. Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis.
    Authors: Rosen L, Freeman A, Yang L, Jutivorakool K, Olivier K, Angkasekwinai N, Suputtamongkol Y, Bennett J, Pyrgos V, Williamson P, Ding L, Holland S, Browne S
    J Immunol, 2013;190(8):3959-66.
    Species: Human
    Sample Type: Plasma
    Application: Bioassay
  57. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors.
    Authors: Doulatov S, Vo L, Chou S, Kim P, Arora N, Li H, Hadland B, Bernstein I, Collins J, Zon L, Daley G
    Cell Stem Cell, 2013;13(4):459-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Endogenous erythroid colony formation in chronic myeloid leukemia: a recurrent finding associated with persistent minimal residual disease under imatinib.
    Authors: Einwallner E, Jaeger E, Mitterbauer-Hohendanner G, Bilban M, Simonitsch-Klupp I, Steiner I, Pernicka E, Hoermann G, Herndlhofer S, Sillaber C, Valent P, Schwarzinger I
    Stem Cells Dev, 2013;22(23):3043-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  59. MHC class II expression in human basophils: induction and lack of functional significance.
    Authors: Voskamp A, Prickett S, Mackay F, Rolland J, O'Hehir R
    PLoS ONE, 2013;8(12):e81777.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  60. Curcumin down-regulates DNA methyltransferase 1 and plays an anti-leukemic role in acute myeloid leukemia.
    Authors: Yu, Jianhua, Peng, Yong, Wu, Lai-Chu, Xie, Zhiliang, Deng, Youcai, Hughes, Tiffany, He, Shun, Mo, XiaoKui, Chiu, Ming, Wang, Qi-En, He, Xiaoming, Liu, Shujun, Grever, Michael, Chan, Kenneth, Liu, Zhongfa
    PLoS ONE, 2013;8(2):e55934.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  61. Tropism of avian influenza A (H5N1) virus to mesenchymal stem cells and CD34+ hematopoietic stem cells.
    Authors: Thanunchai, Maytawan, Kanrai, Pumaree, Wiboon-Ut, Suwimon, Puthavathana, Pilaipan, Hongeng, Suradej, Thitithanyanont, Arunee
    PLoS ONE, 2013;8(12):e81805.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  63. Chronic immune activation in HIV-1 infection contributes to reduced interferon alpha production via enhanced CD40:CD40 ligand interaction.
    Authors: Donhauser N, Pritschet K, Helm M, Harrer T, Schuster P, Ries M, Bischof G, Vollmer J, Smola S, Schmidt B
    PLoS ONE, 2012;7(3):e33925.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  64. Stromal cells expressing hedgehog-interacting protein regulate the proliferation of myeloid neoplasms.
    Blood Cancer J, 2012;2(0):e87.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  65. Neurotensin and CRH interactions augment human mast cell activation.
    Authors: Alysandratos K, Asadi S, Angelidou A, Zhang B, Sismanopoulos N, Yang H, Critchfield A, Theoharides T
    PLoS ONE, 2012;7(11):e48934.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
    Authors: Li Q, Ramirez-Bergeron D, Dunwoodie S, Yang Y
    J Biol Chem, 2012;287(34):29088-100.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  67. Targeting of CD34+CD38- cells using Gemtuzumab ozogamicin (Mylotarg) in combination with tipifarnib (Zarnestra) in Acute Myeloid Leukaemia.
    BMC Cancer, 2012;12(0):431.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
    Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K
    J. Invest. Dermatol., 2011;132(1):114-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  69. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  70. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome).
    Authors: Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B
    Blood, 2011;117(3):839-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  71. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells.
    Authors: Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF
    Gene Ther., 2011;18(11):1078-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011;6(7):e22261.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  73. ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor.
    Authors: Torrano V, Procter J, Cardus P
    Blood, 2011;118(18):4910-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  74. Dynamic interaction networks in a hierarchically organized tissue.
    Authors: Kirouac DC, Ito C, Csaszar E, Roch A, Yu M, Sykes EA, Bader GD, Zandstra PW
    Mol. Syst. Biol., 2010;6(0):417.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  75. IL-3 inhibits human osteoclastogenesis and bone resorption through downregulation of c-Fms and diverts the cells to dendritic cell lineage.
    Authors: Gupta N, Barhanpurkar AP, Tomar GB
    J. Immunol., 2010;185(4):2261-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells.
    Authors: Ghirelli C, Zollinger R, Soumelis V
    Blood, 2010;115(24):5037-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
    Authors: Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
    Blood, 2009;114(24):5091-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009;125(5):1092-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
    Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  80. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15.
    Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS
    Exp. Hematol., 2008;36(5):598-608.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  81. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  82. Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation.
    Authors: Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N
    Exp. Hematol., 2008;36(5):545-558.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  83. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006;13(10):1686-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  85. Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells.
    Authors: Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS
    Allergy, 2006;61(6):769-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006;36(7):1975-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  87. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
    Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
    Exp. Hematol., 2006;34(10):1312-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  88. Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
    Authors: Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y
    Exp. Hematol., 2006;34(6):760-9.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  89. CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
    Authors: Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F
    J. Immunol., 2006;177(1):77-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  90. Thrombospondin 1, produced by endothelial cells under the action of erythropoietin, stimulates thymidine incorporation into erythroid cells and counteracts the inhibitory action of insulin-like growth factor binding protein 3.
    Authors: Congote LF, DiFalco MR, Gibbs BF
    Cytokine, 2005;30(5):248-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  91. Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines.
    Authors: Femenia F, Arock M, Leriche L, Delouis C, Millet G, Ben Hamouda N, Cote M, Alliot A, Lilin T, Pinton A, Iannucceli N, Parodi AL, Boireau P
    Life Sci., 2005;77(7):808-23.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  92. Growth hormone-induced stimulation of multilineage human hematopoiesis.
    Authors: Hanley MB, Napolitano LA, McCune JM
    Stem Cells, 2005;23(8):1170-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  93. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  94. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, Miyamoto T, Akashi K, Weissman IL
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7.
    Species: Human
    Sample Type:
    Application: Bioassay
  95. Expression of functional interleukin-3 receptors on Hodgkin and Reed-Sternberg cells.
    Authors: Aldinucci D, Poletto D, Gloghini A, Nanni P, Degan M, Perin T, Ceolin P, Rossi FM, Gattei V, Carbone A, Pinto A
    Am. J. Pathol., 2002;160(2):585-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  96. Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells.
    Authors: Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG
    J. Leukoc. Biol., 2002;72(4):837-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  97. Genetically modified CD34+ cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model.
    Authors: Gomez-Navarro J, Contreras JL, Arafat W, Jiang XL, Krisky D, Oligino T, Marconi P, Hubbard B, Glorioso JC, Curiel DT, Thomas JM
    Gene Ther., 2000;7(1):43-52.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  98. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  99. Expression of Fas/CD95 and Bcl-2 by primitive hematopoietic progenitors freshly isolated from human fetal liver.
    Authors: Barcena A, Park SW, Banapour B, Muench MO, Mechetner E
    Blood, 1996;88(6):2013-25.
    Species: Human
    Sample Type:
    Application: Bioassay
  100. Proliferating dendritic cell progenitors in human blood.
    Authors: Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman R, Schuler G
    J Exp Med, 1994;180(1):83-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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Animal-Free Recombinant Proteins
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Recombinant Human IL-3, Animal-Free Protein

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