Human CD34 Antibody

Catalog # Availability Size / Price Qty
MAB7227-100
MAB7227-SP
Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human CD34 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISAs
Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human endothelial vesicles
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD34 antibody in KG-1a Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

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Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citations for Human CD34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tunable control of CAR T cell activity through tetracycline mediated disruption of protein-protein interaction
    Authors: A Hotblack, EK Kokalaki, MJ Palton, GW Cheung, IP Williams, S Manzoor, TI Grothier, A Piapi, V Fiaccadori, P Wawrzyniec, HA Roddy, G Agliardi, C Roddie, S Onuoha, S Thomas, S Cordoba, M Pule
    Scientific Reports, 2021;11(1):21902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Immunohistochemical Comparison of the Expression of CD34 and CD105 in Odontogenic Keratocyst and Dentigerous Cyst.
    Authors: Jamshidi S, Zargaran M, Roshanaei G, Hadadi F, Dehghani Nazhvani A
    J Dent (Shiraz), 0;18(1):43-46.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD34 Antibody

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Human CD34 Antibody
By Anonymous on 11/18/2017
Application: Flow Sample Tested: Bone marrow cells Species: Human

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