Human CD34 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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CD34 in Human Liver via seqIF™ staining on COMET™ CD34 was detected in immersion fixed paraffin-embedded sections of human Liver using Mouse Anti-Human CD34, Monoclonal Antibody (Catalog #MAB7227) at 10ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD34 in Human Colon Cancer. CD34 was detected in immersion fixed paraffin sections of human colon cancer using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of CD34 in Human Liver. CD34 was detected in immersion fixed paraffin sections of human liver using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of CD34 in Human Placenta. CD34 was detected in immersion fixed paraffin sections of human placenta using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.Citations for Human CD34 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Tunable control of CAR T cell activity through tetracycline mediated disruption of protein-protein interaction
Authors: A Hotblack, EK Kokalaki, MJ Palton, GW Cheung, IP Williams, S Manzoor, TI Grothier, A Piapi, V Fiaccadori, P Wawrzyniec, HA Roddy, G Agliardi, C Roddie, S Onuoha, S Thomas, S Cordoba, M Pule
Scientific Reports, 2021-11-09;11(1):21902.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immunohistochemical Comparison of the Expression of CD34 and CD105 in Odontogenic Keratocyst and Dentigerous Cyst.
Authors: Jamshidi S, Zargaran M, Roshanaei G, Hadadi F, Dehghani Nazhvani A
J Dent (Shiraz), 2017-03-01;18(1):43-46.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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