Recombinant Human IL-3 Protein, Animal-Free New

Name: Recombinant Human IL-3 Protein, Animal-Free
Brand: R&D Systems
SKU: BT-003-AFL
Price: 594 USD

Catalog #: BT-003-AFL Datasheet / COA / SDS

GMP Version Available: BT-003-GMP

GMP

Catalog #	Availability	Size / Price	Qty
BT-003-AFL-050
BT-003-AFL-01M

Save 15% on Select RUO Reagents. See Details.

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human IL-3.

3 Images

All IL-3 Proteins and Enzymes

Product Details

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-3 Protein, Animal-Free Summary

Learn more about Animal-Free Recombinant Proteins

Product Specifications

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.015-0.150 ng/mL.
The specific activity of recombinant human IL-3 is >1.7 x 10⁶ IU/mg, which is calibrated against the human IL-3 WHO International Standard (NIBSC code: 91/510).

Source

E. coli-derived human IL-3 protein
Ala20-Phe152, with and without an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

N-terminal Sequence
Analysis

Ala20 and Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

14 kDa, under reducing conditions.

Product Datasheets

BT-003-AFL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-003-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

Equivalent bioactivity of GMP (BT-003-GMP), Animal-Free (Catalog # BT-003-AFL) and RUO (203-IL) grades of Recombinant Human IL-3 as measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. (orange, green, red, respectively).

Bioactivity

View Larger

Animal-Free™ Recombinant Human IL‑3 Protein (Catalog # BT-003-AFL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is 0.015-0.150 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Animal-Free™ Recombinant Human IL‑3 Protein (Catalog # BT-003-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 14 kDa under reducing conditions.

Reconstitution Calculator

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Long Name

Interleukin 3

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Alternate Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement