Recombinant Human Thrombopoietin/TPO GMP Protein, CF

Catalog #: BT-TPO-GMP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BT-TPO-GMP-050
BT-TPO-GMP-01M

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Recombinant Human Thrombopoietin/TPO GMP Protein Bioactivity.

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All Thrombopoietin/Tpo Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Thrombopoietin/TPO GMP Protein, CF Summary

TPO Manufactured in Bio-Techne's new GMP facility
Lot-to-lot consistency
Stringent guidelines for patient safety
Scalability necessary to support successful therapeutics
Learn more about manufacturing in our new GMP facility
Test it in your process! Request a sample of GMP TPO

Product Specifications

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining. 18732 Da ± 50 Da

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.0500-0.500 ng/mL.

The specific activity of Recombinant Human Thrombopoietin is >1.00 x 10⁷ units/mg, which is calibrated against the Human Thrombopoietin reference standard (NIBSC code: 03/124).

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22 - Leu195 with an N-terminal Alanine
Produced using non-animal reagents in an animal-free laboratory.

Manufactured and tested under cGMP guidelines.

Accession #

NP_000451.1

N-terminal Sequence
Analysis

Ala-Ser22-Pro-Ala-Pro-Pro-Ala-(Cys)-Asp-Leu

Predicted Molecular Mass

18.7 kDa

SDS-PAGE

18-19 kDa, under reducing conditions.

Product Datasheets

BT-TPO-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-TPO-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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The bioactivity of Recombinant Human Thrombopoietin/TPO GMP Protein (Catalog # BT-TPO-GMP) was measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀for this effect is 0.0500‑0.500 ng/mL. Three independent lots were tested for bioactivity and plotted on the same graph to show lot-to-lot consistency of GMP Thrombopoietin/Tpo protein.

Bioactivity

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Equivalent bioactivity of GMP (Catalog # BT-TPO-GMP) and Animal-Free (BT-TPO-AFL) grades of Recombinant Human Thrombopoietin/TPO as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells (orange and green, respectively).

SDS-PAGE

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2 μg/lane of Recombinant Human Thrombopoietin/TPO GMP Protein (Catalog # BT-TPO-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-19 kDa under reducing conditions.

Data

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Purified CD34+ cells derived from plerixafor mobilized peripheral blood were cultured in commercially available media. The media was supplemented with SCF (100 ng/mL, Catalog # BT-SCF-GMP), TPO (100 ng/mL, Catalog # BT-TPO-GMP), FLT-3L (100 ng/mL, Catalog # BT-FT3L-GMP), and SR1 (1 µM, Catalog # 7086). After 7 days, fold expansion was calculated from total viable cell counts. Frequency (%) CD34+ cells was analyzed by flow cytometry on Days 0 and 7. The average fold expansion of n=2 technical replicates is shown.

Data

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Purified CD34+ cells derived from plerixafor mobilized peripheral blood were cultured in commercially available media. The media was supplemented with SCF (Catalog # BT-SCF-GMP, 100 ng/mL), TPO (Catalog # BT-TPO-GMP, 100 ng/mL), FLT-3L (Catalog # BT-FT3L-GMP, 100 ng/mL), and SR1 (Catalog # 7086, 1 µM). After 7 days, fold expansion was calculated from total viable cell counts. Percent (%) CD34+ cells was analyzed by flow cytometry on Days 0 and 7. The average fold expansion of n=3 technical replicates is shown.

Reconstitution Calculator

Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Alternate Names

Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented and controlled manufacturing process
Control of documentation and process changes by QA
Personnel training programs
Raw material inspection and vendor qualification/monitoring program
Validated equipment, processes and test methods
Equipment calibration and maintenance schedules using a Regulatory Asset Manager
Facility/Utilities maintenance, contamination controls, safety and pest control programs
Material review process for variances
Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

N-terminal amino acid analysis
SDS-PAGE purity analysis
Molecular weight analysis via mass spectrometry
Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
Bioassay analysis
Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
Host cell protein assessment
Host cell DNA assessment
Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production