Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein, CF

(3 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for for this effect is 1.5-9 ng/mL.
  • Source
    E. coli-derived Ser18-Asp169
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser18
  • Predicted Molecular Mass
    17 kDa
  • SDS-PAGE
    17 kDa, reducing coditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6835-IL/CF
 
6835-IL
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in Water.
Reconstitution Reconstitute at 200 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-36 gamma/IL-1F9 (Catalog #
6835-IL/CF) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 1.5-9 ng/mL.
1 µg/lane of Recombinant Human IL-36 gamma /IL-1F9 (aa 18-169) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Background: IL-36 gamma/IL-1F9
IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36 Ra (IL‑1F5), IL-36 alpha (IL‑1F6), IL‑36 beta (IL‑1F8), IL‑37 (IL‑1F7) and IL-1F10 (1‑5). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene (2, 3). IL‑36 gamma is an 18‑22 kDa, 169 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation sites (1, 2, 4, 6, 7). Human IL‑36 gamma (aa 18‑169) shares 58%, 59%, 68% and 69% aa sequence identity with mouse, rat, bovine and equine IL‑36 gamma, respectively, and 23‑57% aa sequence identity with other family members. A 134 aa isoform missing aa 19‑53 has been reported (8). Highest levels of IL‑36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first‑line defense against pathogens in the skin, lungs and digestive tract (2, 3, 6, 9). Its expression is induced by LPS treatment of monocytes, and by IL‑ alpha / beta, IL‑17 or TNF‑ alpha treatment of keratinocytes and bronchial epithelia (1, 6, 7, 9‑11). Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis (1, 6, 11). It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL‑36 gamma and other IL‑36 proteins contribute to neutrophil influx (4, 7, 10). The receptor for IL-36 gamma is a combination of IL‑1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (4, 7, 9). IL‑36 alpha,  beta  and  gamma all activate NF‑ kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and IL‑36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL‑8 (7, 9, 10).
  • References:
    1. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
    2. Busfield, S.J. et al. (2000) Genomics 66:213.
    3. Dunn, E. et al. (2001) Trends Immunol. 22:533.
    4. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
    5. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
    6. Debets, R. et al. (2001) J. Immunol. 167:1440.
    7. Chustz, R.T. et al. (2010) Am. J. Respir. Cell Mol. Biol. Sept. 24 [Epub ahead of print]
    8. SwissProt Accession # Q9NZH8.
    9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
    10. Ramadas, R.A. et al. (2011) Am. J. Respir. Cell Mol. Biol. 44:134.
    11. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  • Long Name:
    Interleukin 36 gamma/Interleukin 1 Family 9
  • Entrez Gene IDs:
    56300 (Human); 215257 (Mouse); 499744 (Rat)
  • Alternate Names:
    IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Applications
Sample Type
  1. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015;290(39):23997-4006.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.
    Authors: Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H
    Sci Rep, 2015;5(0):8521.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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