Recombinant Human IL-36Ra/IL-1F5 Protein

(3 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma -induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.2-1 μg/mL in the presence of 10 ng/mL of recombinant human IL-36 beta.
  • Source
    E. coli-derived Val2-Asp155
  • Accession #
  • N-terminal Sequence
    Analysis
    Val2
  • Predicted Molecular Mass
    17 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1275-IL
 
1275-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human
IL-36Ra/IL-1F5(Catalog # 1275-IL) inhibits Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induced IL-8 secretionby A431 human epithelial carcinoma cells. The ED50 for this effect is0.2-1 μg/mL.
Background: IL-36Ra/IL-1F5

Human interleukin-36 receptor antagonist [IL-36Ra; previously IL-1F5 and also named FIL-1δ (delta), IL‑1HY1, IL‑1H3, and IL-1L1] is a member of the IL‑1 family of proteins (1 ‑ 6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL-1F5-F10 (7). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that underwent multiple duplications (7). The human IL‑36Ra/IL‑1F5 gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of the IL-1ra gene (2, 3). IL-36Ra/IL-1F5 is synthesized as a 155 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2 - 5). Nevertheless, it appears to be secreted as a 17 kDa monomer (5). There is an alternate start site that potentially gives rise to an alternate splice form (5). The translated product, however, has a premature stop codon, resulting in a truncated 16 aa peptide. Human to mouse, full length IL-1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 50% aa identical to IL-1ra, and 32%, 31%, 35%, 37%, 32% and 42% aa identical to IL-1 beta, IL-36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express
IL‑36Ra/IL‑1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for
IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL-1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is also unclear. It was initially reported to be an antagonist of IL‑36 gamma /IL‑1F9 activity (4, 6). This would be consistent with its hypothesized relationship to
IL‑1ra. Studies, however, find IL-36Ra/IL-1F5 antagonist activity difficult to demonstrate (9).

  • References:
    1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
    2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
    3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
    4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
    5. Barton, J.L. et al. (2000) Eur. J. Immunol. 30:3299.
    6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
    7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
    8. Debets, R. et al. (2001) J. Immunol. 167:1440.
    9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  • Long Name:
    Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
  • Entrez Gene IDs:
    26525 (Human); 54450 (Mouse)
  • Alternate Names:
    FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Applications
Sample Type
  1. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015;290(39):23997-4006.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(8):3001-5.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Binding Assay
Recombinant Proteins
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Bind 872-RP 1
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