Recombinant Human IL-37/IL-1F7 Protein

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED50 for this effect is 0.25-1.25 μg/mL.
  • Source
    E. coli-derived Ser2-Asp192
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser2
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    22 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9225-IL
 
9225-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human
IL-37/IL-1F7(Catalog # 9225-IL) inhibits LPS-stimulated IL-8 secretion by HK-2 human kidneyepithelial cells. The ED50 for this effect is 0.25-1.25 μg/mL.
Background: IL-37/IL-1F7
Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL-37a through e) with deletions in the N-terminal region of the molecule (4-7). IL-37b shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta, and IL-1ra, respectively. Mouse IL-37 has not been reported, but human IL-37b is active on mouse cells. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL-37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL-37b can form homodimers in solution (8, 10). Although IL-37b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (7, 10, 11). Alternatively, IL-37b will also bind to IL-18 BP, generating a complex that interacts with IL-18 R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (11). IL-37b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (12).
  • References:
    1. Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466.
    2. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
    3. Ding, V.A. et al. (2016) Med. Oncol. 33:68.
    4. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
    5. Kumar S. et al. (2000) J. Biol Chem 275:10308.
    6. Busfield S.J. et al. (2000) Genomics 66:213.
    7. Pan, G. et al. (2000) Cytokine 13:1.
    8. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
    9. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
    10. Kumar S. et al. (2002) Cytokine 18:61.
    11. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
    12. Sharma, S. et al. (2008) J. Immunol. 180:5477.
  • Long Name:
    Interleukin 37
  • Entrez Gene IDs:
    27178 (Human)
  • Alternate Names:
    FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37/IL-1F7; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein
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