Recombinant Human IL-4I1 Protein, CF

R&D Systems | Catalog # 5684-AO

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human IL-4I1 Protein (5684-AO)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q96RQ9

Structure / Form

Monomer

Applications

Enzyme Activity
View all IL-4I1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-4I1 protein
Gln22-His567, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

61 kDa

SDS-PAGE

70-75 kDa, reducing conditions

Activity

Measured by its ability to oxidize phenylalanine in a horseradish peroxidase coupled assay.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 5 using 5684-AO in the following applications:

Scientific Data Images for Recombinant Human IL-4I1 Protein, CF

Enzyme Activity of Human IL-4I1 Protein

Recombinant Human IL-4I1 Protein (Catalog # 5684-AO) is measured by its ability to oxidize phenylalanine in a horseradish peroxidase coupled assay.
SDS-PAGE gel picture of purified Recombinant Human IL-4I1 Protein

SDS-PAGE of Human IL-4I1 Protein

2 μg/lane of Recombinant Human IL-4I1 Protein (5684-AO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at ~71 under reducing conditions.

Formulation, Preparation, and Storage

5684-AO
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: IL-4I1

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H2O2 production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).

References

  1. Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
  2. Mason, J.M. et al. (2004) J. Immunol. 173:4561.
  3. Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
  4. Copie-Bergman, C. et al. (2003) Blood 101:2756.
  5. Boulland, M.L. et al. (2007) Blood 110:220.

Long Name

Interleukin 4-induced Protein-1

Alternate Names

FIG1, IL4I1

Entrez Gene IDs

259307 (Human); 14204 (Mouse); 308575 (Rat); 102115096 (Cynomolgus Monkey)

Gene Symbol

IL4I1

UniProt

Q96RQ9

Additional IL-4I1 Products

Product Documents for Recombinant Human IL-4I1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IL-4I1 Protein, CF

For research use only

Citations for Recombinant Human IL-4I1 Protein, CF

Customer Reviews for Recombinant Human IL-4I1 Protein, CF (2)

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  • Recombinant Human IL-4I1 Protein, CF
    Name: Gerre Lafond
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/15/2021
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    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 12/04/2020

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Protocols

View specific protocols for Recombinant Human IL-4I1 Protein, CF (5684-AO):

Materials
  • Assay Buffer: 50 mM Sodium Phosphate, pH 7.0
  • Recombinant Human IL-4I1 (rhIL-4I1) (Catalog # 5684-AO)
  • Coupling Agent: Horseradish Peroxidase (HRP) (250-330 U/mg) (Sigma, Catalog # P8375), 250 units/mL stock in 0.1 M Sodium Phosphate, pH 8.0
  • Substrate Component 1: Phenylalanine (Sigma, Catalog # P2126), 100 mM stock in diH2O
  • Substrate Component 2: Amplex Ultra Red (AUR) (Molecular Probes, Catalog # A36006), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhIL-4I1 to 2 ng/µL in Assay Buffer.
  2. Prepare the Substrate mixture including 2 mM Phenylalanine, 2 units/mL HRP and 100 µM AUR in Assay Buffer.
  3. Load in a black well plate 50 µL of 2 ng/µL of rhIL-4I1, and start the reaction by adding 50 µL of the Substrate mixture (step 2). Include a Substrate Blank containing 50 µL of the Assay Buffer and 50 µL of the Substrate mixture.
  4. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes. Note: A wavelength cutoff of 570 nm should be manually specified.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

*Adjusted for Substrate Blank
**Derived using a fluorescent standard prepared by incubating 50 µM AUR, 1 unit/mL HRP, 1 mM Phenylalanine, and a curve of Hydrogen Peroxide (Sigma, Catalog # H1009) in Assay Buffer at room temperature. Use the resulting amounts of oxidized AUR to determine the conversion factor.

Per Well:
  • rhIL-4I1: 0.1 µg
  • Phenylalanine: 1 mM
  • HRP: 1 unit/mL
  • AUR: 50 µM

Caution: The rhIL-4I1 enzymatic reaction may significantly be interfered with organic buffer such as MES.

FAQs

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