Recombinant Human Integrin alpha 4 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
5668-A4-050
Product Details
Citations (4)
FAQs
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Recombinant Human Integrin alpha 4 beta 1 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 104 cells per well are added to rhIntegrin alpha 4 beta 1 coated plates (10 μg/mL,
100 μL/well), approximately 45%-75% will adhere after 1 hour at 37 °C.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 4 beta 1 protein
Human Integrin alpha 4
(Tyr34-Gln970 (Arg591Leu & Arg878Gln))
Accession # P13612
Acidic Tail 6-His tag
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Tyr34 ( alpha 4) & No results obtained: Gln21predicted ( beta 1)
Predicted Molecular Mass
108.8 kDa ( alpha 4) & 82.3 kDa ( beta 1)
SDS-PAGE
100-150 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5668-A4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha 4 beta 1

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with integrin alpha 4 beta 7 (1 - 4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2 - 4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa human alpha 4 extracellular domain (ECD) shares 85% aa identity with mouse, rat and canine alpha 4, while the 708 aa human beta 1 ECD shares 92 - 96% aa identity with rat, bovine, mouse, and feline beta 1. Five alternate splice forms of the human beta 1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (6 - 10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (11 - 13). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (13, 14).

References
  1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Takada, Y. et al. (2007) Genome Biol. 8:215.
  5. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  6. Eshghi, S. et al. (2007) J. Cell Biol. 177:871.
  7. Yang, J.T. et al. (1995) Development 121:549.
  8. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27.
  9. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38.
  10. Grazioli, A. et al. (2006) Dev. Biol. 293:165.
  11. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525.
  12. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920.
  13. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004.
  14. Nguyen, K. et al. (2008) Immunity 28:810.
Entrez Gene IDs
3676 (Human)
Alternate Names
Integrin alpha 4 beta 1

Citations for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding
    Authors: EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick
    MBio, 2018;9(3):.
    Applications: Bioassay
  2. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
    Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
    PLoS Pathog., 2018;14(8):e1007278.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET.
    Authors: Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A
    PLoS ONE, 2015;10(3):e0121399.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding.
    Authors: Azab W, Zajic L, Osterrieder N
    Vet Res, 2012;43(1):61.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay

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