Best Seller

Recombinant Human Integrin alpha 4 beta 1 Protein, CF

R&D Systems | Catalog # 5668-A4

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 4 beta 1 Protein (5668-A4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P13612 (Integrin alpha 4) & P05556 (Integrin beta 1)

Applications

Bioactivity
View all Integrin alpha 4 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 4 beta 1 protein
Human Integrin alpha 4
(Tyr34-Gln970 (Arg591Leu & Arg878Gln))
Accession # P13612		 Acidic Tail 6-His tag
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556		 Basic Tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr34 ( alpha 4) & No results obtained: Gln21predicted ( beta 1)

Predicted Molecular Mass

108.8 kDa ( alpha 4) & 82.3 kDa ( beta 1)

SDS-PAGE

100-150 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.
When 5 x 104 cells per well are added to rhIntegrin alpha 4 beta 1 coated plates (10 μg/mL,
100 μL/well), approximately 45%-75% will adhere after 1 hour at 37 °C.

Formulation, Preparation, and Storage

5668-A4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Integrin alpha 4 beta 1

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with integrin alpha 4 beta 7 (1 - 4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2 - 4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa human alpha 4 extracellular domain (ECD) shares 85% aa identity with mouse, rat and canine alpha 4, while the 708 aa human beta 1 ECD shares 92 - 96% aa identity with rat, bovine, mouse, and feline beta 1. Five alternate splice forms of the human beta 1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (6 - 10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (11 - 13). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (13, 14).

References

  1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Takada, Y. et al. (2007) Genome Biol. 8:215.
  5. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  6. Eshghi, S. et al. (2007) J. Cell Biol. 177:871.
  7. Yang, J.T. et al. (1995) Development 121:549.
  8. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27.
  9. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38.
  10. Grazioli, A. et al. (2006) Dev. Biol. 293:165.
  11. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525.
  12. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920.
  13. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004.
  14. Nguyen, K. et al. (2008) Immunity 28:810.

Alternate Names

CD49d, IA4

Entrez Gene IDs

3676 (Human)

Gene Symbol

ITGA4

UniProt

P13612 (Integrin alpha 4) & P05556 (Integrin beta 1)

Additional Integrin alpha 4 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

Customer Reviews for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Integrin alpha 4 beta 1 Protein, CF and earn rewards!

Have you used Recombinant Human Integrin alpha 4 beta 1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

Showing  1 - 1 of 1 FAQ Showing All

  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Proteins and Enzymes