Recombinant Human Integrin alpha V beta 3 Protein, CF

Catalog # Availability Size / Price Qty
3050-AV-050

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Recombinant Human Integrin alpha V beta 3 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin (Catalog # 2308-VN) at 1 µg/mL can bind Recombinant Human Integrin alpha V beta 3 with an apparent KD <10 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 3 protein
Human Integrin alpha V subunit
(Phe31-Val992)
Accession # NP_002201
His-Pro GGGSGGGS  Acidic Tail
Human Integrin beta 3 subunit
(Gly27-Asp718)
Accession # AAA52589
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe31 & Gly27
Predicted Molecular Mass
110.5 kDa ( alpha V subunit), 80.8 kDa ( beta 3 subunit)
SDS-PAGE
105-150 kDa, reducing conditions

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3050-AV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3050-AV

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Integrin alpha V beta 3

Integrin  alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non‑covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N‑terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta‑propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine‑rich I‑EGF folds. The alpha V subunit domains termed thigh, calf‑1 and calf‑2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). The 962 aa human alpha V ECD(11) shares 92‑95% aa sequence identity with mouse, rat and bovine  alpha V while the 685 aa human beta 3 ECD(12) shares 95% aa identity with equine and canine, and 89‑92% aa identity with mouse, rat and porcine beta 3. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M‑CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF‑1 to promote cancer cell proliferation and invasiveness (13, 14). Also, cell entry of several viruses is mediated by alpha V beta 3 (4, 10).

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:69.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
  13. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  14. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.
Entrez Gene IDs
3685 (Human)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142

Citations for Recombinant Human Integrin alpha V beta 3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alphavbeta3 Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies
    Authors: A Bugatti, F Filippini, M Bardelli, A Zani, P Chiodelli, S Messali, A Caruso, F Caccuri
    Viruses, 2022;14(4):.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  2. Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist
    Authors: EG Norris, XS Pan, DC Hocking
    bioRxiv : the preprint server for biology, 2022;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  3. Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
    Authors: S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser
    EBioMedicine, 2021;72(0):103617.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  4. The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
    Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
    PloS Pathogens, 2021;17(12):e1010083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44
    Authors: G Fnu, P Agrawal, GC Kundu, GF Weber
    Biomolecules, 2021;11(6):.
    Species: Human
    Sample Types: Recombinant Protein
  6. Engineering bacterial outer membrane vesicles as transdermal nanoplatforms for photo-TRAIL-programmed therapy against melanoma
    Authors: LH Peng, MZ Wang, Y Chu, L Zhang, J Niu, HT Shao, TJ Yuan, ZH Jiang, JQ Gao, XH Ning
    Sci Adv, 2020;6(27):eaba2735.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity
    Authors: A Szlachcic, M Sochacka, A Czyrek, L Opalinski, D Krowarsch, J Otlewski, M Zakrzewska
    Cells, 2019;8(8):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Pull Down Assay
  8. Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
    Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
    J. Clin. Invest., 2019;129(4):1713-1726.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  9. Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis
    Authors: S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber
    Sci Rep, 2019;9(1):4661.
    Species: Human
    Sample Types: Imaging Agent
    Applications: Binding Assay
  10. Integrin targeting of glyphosate and its cell adhesion modulation effects on osteoblastic MC3T3-E1 cells revealed by label-free optical biosensing
    Authors: I Szekacs, E Farkas, BL Gemes, E Takacs, A Szekacs, R Horvath
    Sci Rep, 2018;8(1):17401.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and ?v?3 integrin for osteoporosis therapy
    Authors: Y Zur, L Rosenfeld, CA Keshelman, N Dalal, G Guterman-R, A Orenbuch, Y Einav, N Levaot, N Papo
    PLoS Biol., 2018;16(8):e2002979.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
    Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
    Sci Rep, 2017;7(1):3185.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat.
    Authors: Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T
    J Biol Chem, 2015;290(42):25534-47.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. SAA1 polymorphisms are associated with variation in antiangiogenic and tumor-suppressive activities in nasopharyngeal carcinoma.
    Authors: Lung H, Man O, Yeung M, Ko J, Cheung A, Law E, Yu Z, Shuen W, Tung E, Chan S, Bangarusamy D, Cheng Y, Yang X, Kan R, Phoon Y, Chan K, Chua D, Kwong D, Lee A, Ji M, Lung M
    Oncogene, 2015;34(7):878-89.
  15. Quantity and accessibility for specific targeting of receptors in tumours.
    Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
    Sci Rep, 2014;4(0):5232.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
    Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
    PLoS ONE, 2013;8(11):e81165.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  17. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012;31(0):26.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  18. Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
    Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
    Gene Ther., 2009;16(12):1405-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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Reviews for Recombinant Human Integrin alpha V beta 3 Protein, CF

Average Rating: 4 (Based on 2 Reviews)

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Recombinant Human Integrin alpha V beta 3 Protein, CF
By Anonymous on 05/04/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: Stable molecule that gives reproducible results even after long term (a few hours) incubation at 37C

Recombinant Human Integrin alpha V beta 3 Protein, CF
By Zhi - Ren Liu on 05/18/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: Very low shelf life, buffer conditions are not very optimistic.

Integrin avb3 we purchased was good, but they wouldn't separate on reducing gel, the heterodimer remain strongly intact. I have used for binding assays, and in SPR it worked very well. traces of calcium might be present which inhibits integrin avb3 but has to be washed completely removing those ions.