Recombinant Human Jagged 1 Fc Chimera Protein, CF

  (20 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
    Human Jagged 1
    (Ser32-Ser1046)
    Accession # P78504
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser32
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    137 kDa
  • SDS-PAGE
    170-180 kDa, under reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

1277-JG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
 
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

  • References:
    1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
    2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
    3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
    4. Lai, E.C. (2004) Development 131:965.
    5. Milner, L.A. et al. (1994) Blood 83:2057.  
    6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
    7. Linheng, L. et al. (1998) Immunity 8:43.
    8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
    9. Oda, T. et al. (1997) Nat. Genet. 16:235.
    10. Oda, T. et al. (1997) Genomics 43:376.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. RNA sequencing data of Notch ligand treated human dental pulp cells
    Authors: J Manokawinc, P Nattasit, T Thongngam, P Pavasant, KA Tompkins, H Egusa, T Osathanon
    Data Brief, 2018;17(0):407-413.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Sumoylation of Notch1 represses its target gene expression during cell stress
    Authors: CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren
    Cell Death Differ., 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1? Upstream of Notch and SUMO Pathways
    Authors: M Ciria, NA García, I Ontoria-Ov, H González-K, R Carrero, JL De La Pomp, JA Montero, P Sepúlveda
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration
    Authors: Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
    Authors: T Gocha, BM Rao, R DasGupta
    Sci Rep, 2017;7(1):12021.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration
    Authors: X Hong, A Margariti, A Le Bras, L Jacquet, W Kong, Y Hu, Q Xu
    Sci Rep, 2017;7(1):5590.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
    Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
    PLoS ONE, 2016;11(1):e0148216.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Jagged1 Instructs Macrophage Differentiation in Leprosy
    PLoS Pathog, 2016;12(8):e1005808.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Notch1 signaling regulates the epithelial-mesenchymal transition and invasion of breast cancer in a Slug-dependent manner.
    Authors: Shao, Shan, Zhao, Xiaoai, Zhang, Xiaojin, Luo, Minna, Zuo, Xiaoxiao, Huang, Shangke, Wang, Ying, Gu, Shanzhi, Zhao, Xinhan
    Mol Cancer, 2015;14(1):28.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015;17(9):1193-204.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche.
    Authors: Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A
    J Transl Med, 2015;13(0):27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. 3D niche microarrays for systems-level analyses of cell fate.
    Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
    Nat Commun, 2014;5(0):4324.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  13. Augmented osteogenic responses in human aortic valve cells exposed to oxLDL and TLR4 agonist: a mechanistic role of Notch1 and NF-kappaB interaction.
    Authors: Zeng Q, Song R, Ao L, Xu D, Venardos N, Fullerton D, Meng X
    PLoS ONE, 2014;9(5):e95400.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
    Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
    Nat Commun, 2014;5(0):4806.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Notch signaling is involved in neurogenic commitment of human periodontal ligament-derived mesenchymal stem cells.
    Authors: Osathanon T, Manokawinchoke J, Nowwarote N, Aguilar P, Palaga T, Pavasant P
    Stem Cells Dev, 2013;22(8):1220-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Notch1 stimulation induces a vascularization switch with pericyte-like cell differentiation of glioblastoma stem cells.
    Authors: Guichet P, Guelfi S, Teigell M, Hoppe L, Bakalara N, Bauchet L, Duffau H, Lamszus K, Rothhut B, Hugnot J
    Stem Cells, 2013;33(1):21-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012;7(7):e40490.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis.
    Authors: Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM
    Circulation, 2012;123(3):309-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012;181(3):1099-111.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

Human IgG1 Antibody

WB , ELISA MAB9894  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Human IgG1 Antibody

WB MAB98941  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human IgG1 Fc Protein, CF

BA 110-HG 72
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Recombinant Human Jagged 1 Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Recombinant Human Jagged 1 Fc Chimera Protein, CF.

We have 1 review tested in 1 application: In vitro bioactivity in cell culture.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
In vitro bioactivity in cell culture
(1)
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
In vitro bioactivity in cell culture Recombinant Human Jagged 1 Fc Chimera Protein, CF 1277-JG Array 1277-JG
Enlarge
Excellent
 In vitro bioactivity in cell culture Anonymous 04/04/2018
Show
Image Details
In vitro bioactivity in cell culture Recombinant Human Jagged 1 Fc Chimera Protein, CF 1277-JG
In vitro bioactivity in cell culture: Recombinant Human Jagged 1 Fc Chimera Protein, CF [1277-JG].

Summary

Application In vitro bioactivity in cell culture
Reason for Rating Good performance in Notch-reporter assay in cell lines

Other Experimental Details

Other Experimental Details Target cells were transiently transfected with a luciferase responsive Notch promoter (CSL) as well as a constitutively expressed Renilla plasmids. Prot G followed by IgG or Jag1 chimera incubations on 96-well plates. Target cells seeded onto these wells, and DMSO or DAPT (a Notch inhibitor) added.

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee