Recombinant Human Jagged 1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1277-JG-050
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
137 kDa
SDS-PAGE
170-180 kDa, under reducing conditions

Product Datasheets

1277-JG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Citations for Recombinant Human Jagged 1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. RNA sequencing data of Notch ligand treated human dental pulp cells
    Authors: J Manokawinc, P Nattasit, T Thongngam, P Pavasant, KA Tompkins, H Egusa, T Osathanon
    Data Brief, 2018;17(0):407-413.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Sumoylation of Notch1 represses its target gene expression during cell stress
    Authors: CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren
    Cell Death Differ., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration
    Authors: X Hong, A Margariti, A Le Bras, L Jacquet, W Kong, Y Hu, Q Xu
    Sci Rep, 2017;7(1):5590.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration
    Authors: Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1? Upstream of Notch and SUMO Pathways
    Authors: M Ciria, NA García, I Ontoria-Ov, H González-K, R Carrero, JL De La Pomp, JA Montero, P Sepúlveda
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
    Authors: T Gocha, BM Rao, R DasGupta
    Sci Rep, 2017;7(1):12021.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
    Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
    PLoS ONE, 2016;11(1):e0148216.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Jagged1 Instructs Macrophage Differentiation in Leprosy
    PLoS Pathog, 2016;12(8):e1005808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche.
    Authors: Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A
    J Transl Med, 2015;13(0):27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Notch1 signaling regulates the epithelial-mesenchymal transition and invasion of breast cancer in a Slug-dependent manner.
    Authors: Shao, Shan, Zhao, Xiaoai, Zhang, Xiaojin, Luo, Minna, Zuo, Xiaoxiao, Huang, Shangke, Wang, Ying, Gu, Shanzhi, Zhao, Xinhan
    Mol Cancer, 2015;14(1):28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015;17(9):1193-204.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Augmented osteogenic responses in human aortic valve cells exposed to oxLDL and TLR4 agonist: a mechanistic role of Notch1 and NF-kappaB interaction.
    Authors: Zeng Q, Song R, Ao L, Xu D, Venardos N, Fullerton D, Meng X
    PLoS ONE, 2014;9(5):e95400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. 3D niche microarrays for systems-level analyses of cell fate.
    Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
    Nat Commun, 2014;5(0):4324.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  14. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
    Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
    Nat Commun, 2014;5(0):4806.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Notch1 stimulation induces a vascularization switch with pericyte-like cell differentiation of glioblastoma stem cells.
    Authors: Guichet P, Guelfi S, Teigell M, Hoppe L, Bakalara N, Bauchet L, Duffau H, Lamszus K, Rothhut B, Hugnot J
    Stem Cells, 2013;33(1):21-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Notch signaling is involved in neurogenic commitment of human periodontal ligament-derived mesenchymal stem cells.
    Authors: Osathanon T, Manokawinchoke J, Nowwarote N, Aguilar P, Palaga T, Pavasant P
    Stem Cells Dev, 2013;22(8):1220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012;181(3):1099-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis.
    Authors: Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM
    Circulation, 2012;123(3):309-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012;7(7):e40490.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human Jagged 1 Fc Chimera Protein, CF

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Recombinant Human Jagged 1 Fc Chimera Protein, CF
By Anonymous on 04/04/2018
Application: In vitro bioactivity in cell culture
Reason for Rating: Good performance in Notch-reporter assay in cell lines

Target cells were transiently transfected with a luciferase responsive Notch promoter (CSL) as well as a constitutively expressed Renilla plasmids. Prot G followed by IgG or Jag1 chimera incubations on 96-well plates. Target cells seeded onto these wells, and DMSO or DAPT (a Notch inhibitor) added.