Recombinant Human Kallikrein 12 Protein, CF

R&D Systems | Catalog # 3095-SE

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human Kallikrein 12 Protein (3095-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_665901

Structure / Form

Pro form

Applications

Enzyme Activity
View all Kallikrein 12 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Kallikrein 12 protein
Ala18-Asn248, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

26 kDa

SDS-PAGE

37 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is > 4,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

3095-SE
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 12

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family. It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (Catalog # http://www.rndsystems.com/product_results.aspx?k=1470-PI">1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The KLK12 produced by the R&D Systems corresponds to the full-length classical form, also known as isoform 2 (1). The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat. The recombinant human KLK12 can be autoactivated under the conditions described in the Activity Assay Protocol. The active enzyme has the N-terminal sequence of I22FNGTECGRNS.

References

  1. Yousef, G.M. et al. (2000) Genomics. 69:331.
  2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Alternate Names

KLK-L5, KLK12

Entrez Gene IDs

43849 (Human)

Gene Symbol

KLK12

UniProt

NP_665901

Additional Kallikrein 12 Products

Product Documents for Recombinant Human Kallikrein 12 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Kallikrein 12 Protein, CF

For research use only

Citations for Recombinant Human Kallikrein 12 Protein, CF

Customer Reviews for Recombinant Human Kallikrein 12 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Kallikrein 12 Protein, CF and earn rewards!

Have you used Recombinant Human Kallikrein 12 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human Kallikrein 12 Protein, CF (3095-SE):

Materials
  • Activation Buffer: 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 8.0
  • Assay Buffer: 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5
  • Recombinant Human Kallikrein 12 (rhKLK12) (Catalog # 3095-SE)
  • Fluorescent Peptide Substrate: BOC-Val-Pro-Arg-AMC (Catalog # ES011), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhKLK12 to 100 µg/mL in Activation Buffer.
  2. Incubate 100 µg/mL rhKLK12 at 37 °C for 24 hours.
  3. Dilute rhKLK12 to 0.2 ng/µL in Assay Buffer.
  4. Dilute Substrate to 200 µM in Assay Buffer.
  5. Load into plate 50 µL of 0.2 ng/µL rhKLK12, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing Assay Buffer in place of rhKLK12.
  6. Read plate at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate Specific Activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).

Per Well:

  • rhKLK12: 0.01 µg
  • Substrate: 100 µM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes