Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

Name: Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11169-LS
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 11169-LS

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human L-Selectin/CD62L Fc Chimera Protein (11169-LS)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAH20758.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all L-Selectin/CD62L Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human L-Selectin/CD62L protein

Human L-Selectin (Trp52-Leu332) Accession # AAH20758.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp52

Predicted Molecular Mass

58 kDa

SDS-PAGE

85-100 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.
The ED₅₀ for this effect is 0.350-3.50 μg/mL.

Scientific Data Images for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein Bioactivity.

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11169-LS) supports the adhesion LS180 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 0.350-3.50 μg/mL.

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11169-LS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Formulation, Preparation, and Storage

11169-LS

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: L-Selectin/CD62L

L-Selectin, also known as Leukocyte adhesion molecule 1 (LAM-1) and CD62L, is a type-1 cell surface glycoprotein and cell adhesion molecule of the Selectin family (1). In humans, there are 3 Selectins, P, E, and L, and they are Ca2+ dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (2). Mature L-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an epidermal growth factor (EGF)-like domain, a transmembrane domain, and a short cytoplasmic domain. Within the ECD, human L-selectin shares 76% and 78% amino acid sequence identity with mouse and rat L-selectin, respectively. Several isoforms arising from alternative splicing have been reported, some with potential therapeutic implications (2,3). L-selectin is constitutively expressed on a wide variety of leukocytes and plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites (1-4). Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium (5). This initial interaction, also involving ICAM‑1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (6). L-selectin function is required for normal Treg cell migration and over expression might be result in reduced tumor growth (7). Several studies have reported that levels of L-Selectin may be elevated or lowered in subjects with a variety of conditions, such as in Alzheimer's disease or rheumatoid arthritis (3, 5).

References

Ivetic, A. et al. (2019) Front. Immunol. 10:1068.
Grailer, J.J. et al. (2009) J. Dermatol sci. 56:141.
Hirata, T. et al. (2015) Biochem Biophys Res. Commun. 462:371.
Wedepohl, S. et al. (2012) Euro. J. Cell Biol. 91:257.
Ivetic, A. et al. (2013) Inter. J. Biochem. Cell Biol. 45:550.
Granger, D.N. and Senchenkova, E. (2010) Inflammation and the Microcirculation. San Rafael (CA): Morgan & Claypool Life Sciences Chapter 7.
Watson, H.A. et al. (2019) Frontiers in immunology 10:1321.