Recombinant Human LDLR Protein, CF

Newer Version Available: 2148-HP
A New and Improved rh LDL R is Now Available! The new protein has ~50% better activity!
Formulations:
Catalog # Availability Size / Price Qty
2148-LD-025/CF
Product Details
Citations (8)
FAQs
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Recombinant Human LDLR Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/mL (100 μL/well), Recombinant Human LDL R inhibits 50% binding of biotinylated recombinant human LDL R (0.5 μg/mL) at the concentration range of 0.4-2 μg/mL
Source
Mouse myeloma cell line, NS0-derived human LDL R protein
Ala22-Arg788, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala22 & Asp193
Predicted Molecular Mass
85.6 kDa & 66.8 kDa
SDS-PAGE
125-140 kDa & 95-110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2148-LD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2148-LD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors (1-5). Members of the family are endocytic receptors, but can also co-regulate adjacent cell-surface signaling molecules (3, 4). Many proteins in the LDL R family are cleaved by extracellular proteases to release soluble forms to the circulation, and many of these soluble forms are active (1, 6). Mature LDL R is a 120-160 kDa (depending on glycosylation) type I transmembrane glycoprotein that contains cysteine-rich complement-like repeats (class A LDL domains), calcium-binding EGF repeats, and beta ‑propeller structures (class B LDL repeats) in the extracellular domain (ECD) (1-7). A membrane-proximal Ser/Thr-rich region shows extensive O-linked glycosylation (4, 8). A cytoplasmic NPxY motif links the LDL R to clathrin pits for endocytosis, and binds to select adaptor proteins (1, 4, 8). The human LDL R ECD shares 78%, 76%, 81% and 82% aa sequence identity with mouse, rat, bovine, and porcine LDL R, respectively. LDL R is constitutively and widely expressed. Its class A LDL domains near the N-terminus bind apoB and apoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively (1, 2, 4, 9). Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol (2, 9). At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface (1, 4).  Lack of LDL R expression or function causes familial hypercholesterolemia (FH) (4, 9, 10). The protease PCSK9 (proprotein convertase subtilisin/kexin type 9) can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface (10-12). Soluble forms of approximately 140 kDa and 28 kDa are reported to be released by phorbol esters or interferons, respectively (6, 7)

References
  1. Go, G.W. and A. Mani (2012) Yale J. Biol. Med. 85:19.
  2. Ren, G. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1059.
  3. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
  4. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
  5. Yamamoto, T. et al. (1984) Cell 39:27.
  6. Begg, M.J. et al. (2004) Eur. J. Biochem. 271:524.
  7. Fischer, D.G. et al. (1993) Science 262:250.
  8. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560.
  9. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
  10. De Castro-Oros, I. et al. (2010) Appl. Clin Genet. 3:53.
  11. Zhang, D.W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13045.
  12. Tavori, H. et al. (2013) Circulation 127:2403.
Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

Citations for Recombinant Human LDLR Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Authors: SA Felt, GN Droby, VZ Grdzelishv
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells.
    Authors: Kraehling J, Chidlow J, Rajagopal C, Sugiyama M, Fowler J, Lee M, Zhang X, Ramirez C, Park E, Tao B, Chen K, Kuruvilla L, Larrivee B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle M, Herz J, Williams K, Eichmann A, Lee W, Fernandez-Hernando C, Sessa W
    Nat Commun, 2016;7(0):13516.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  3. Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
    Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
    Blood, 2014;123(9):1422-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  5. Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII.
    Authors: Kurasawa J, Shestopal S, Karnaukhova E, Struble E, Lee T, Sarafanov A
    J Biol Chem, 2013;288(30):22033-41.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  6. Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor.
    Authors: Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM
    Biochem. J., 2009;419(3):577-84.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  7. Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein.
    Authors: Chien YJ, Chen WJ, Hsu WL, Chiou SS
    Virology, 2008;379(1):143-51.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Effects of pH and low density lipoprotein (LDL) on PCSK9-dependent LDL receptor regulation.
    Authors: Fisher TS, Lo Surdo P, Pandit S, Mattu M, Santoro JC, Wisniewski D, Cummings RT, Calzetta A, Cubbon RM, Fischer PA, Tarachandani A, De Francesco R, Wright SD, Sparrow CP, Carfi A, Sitlani A
    J. Biol. Chem., 2007;282(28):20502-12.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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