Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Catalog # Availability Size / Price Qty
9226-T4-050
Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Bioactivity
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Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 0.3-1.8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6 protein
Pro17-Asn461, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Pro17 & Gly24
Predicted Molecular Mass
49 kDa
SDS-PAGE
62-76 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9226-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

WhenRecombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL,Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) binds with anED50 of
0.3-1.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA1/CD85i/LIR-6

LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).

References
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  4. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  5. Jones, D.C. et al. (2011) J. Immunol. 186:2990.
  6. Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
  7. Zheng, J. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
Entrez Gene IDs
11024 (Human)
Alternate Names
CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LILRA1; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563

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