Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

R&D Systems | Catalog # 9226-T4

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human LILRA1/CD85i/LIR-6 Protein (9226-T4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O75019

Applications

Bioactivity
View all LILRA1/CD85i/LIR-6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6 protein
Pro17-Asn461, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro17 & Gly24

Predicted Molecular Mass

49 kDa

SDS-PAGE

62-76 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 0.3-1.8 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 9226-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Recombinant Human LILRA1/CD85i/LIR-6 Protein Bioactivity

Recombinant Human LILRA1/CD85i/LIR-6 Protein Bioactivity

When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.3-1.8 µg/mL.

Formulation, Preparation, and Storage

9226-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA1/CD85i/LIR-6

LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  4. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  5. Jones, D.C. et al. (2011) J. Immunol. 186:2990.
  6. Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
  7. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1

Alternate Names

CD85i, LIR-6, LIR6

Entrez Gene IDs

11024 (Human)

Gene Symbol

LILRA1

UniProt

O75019

Additional LILRA1/CD85i/LIR-6 Products

Product Documents for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

For research use only

Customer Reviews for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF (1)

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/06/2020
    Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)
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