Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Catalog # Availability Size / Price Qty
9226-T4-050
Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Bioactivity
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Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 0.3-1.8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6 protein
Pro17-Asn461, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Pro17 & Gly24
Predicted Molecular Mass
49 kDa
SDS-PAGE
62-76 kDa, reducing conditions

Product Datasheets

9226-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Bioactivity View Larger

WhenRecombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL,Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) binds with anED50 of
0.3-1.8 µg/mL.

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Background: LILRA1/CD85i/LIR-6

LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).

References
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  4. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  5. Jones, D.C. et al. (2011) J. Immunol. 186:2990.
  6. Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
  7. Zheng, J. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
Entrez Gene IDs
11024 (Human)
Alternate Names
CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LILRA1; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563

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