Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF Summary
Pro17-Asn461, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.3-1.8 µg/mL.
LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).
- Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
- Borges, L. et al. (1997) J. Immunol. 159:5192.
- Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
- Allen, R.L. et al. (2001) J. Immunol. 167:5543.
- Jones, D.C. et al. (2011) J. Immunol. 186:2990.
- Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
- Zheng, J. et al. (2012) Nature 485:656.
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