Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 0.3-1.8 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Pro17-Asn461, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Pro17 & Gly24
  • Predicted Molecular Mass
    49 kDa
  • SDS-PAGE
    62-76 kDa, reducing conditions
9226-T4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
WhenRecombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL,Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) binds with anED50 of
0.3-1.8 µg/mL.
Background: LILRA1/CD85i/LIR-6
LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).
  • References:
    1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
    2. Borges, L. et al. (1997) J. Immunol. 159:5192.
    3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
    4. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
    5. Jones, D.C. et al. (2011) J. Immunol. 186:2990.
    6. Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
    7. Zheng, J. et al. (2012) Nature 485:656.
  • Long Name:
    Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
  • Entrez Gene IDs:
    11024 (Human)
  • Alternate Names:
    CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LILRA1/CD85i/LIR-6; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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