Recombinant Human LILRA5/CD85f/LIR-9 Protein, CF

R&D Systems | Catalog # 8956-T4

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human LILRA5/CD85f/LIR-9 Protein (8956-T4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

A6NI73

Applications

Bioactivity
View all LILRA5/CD85f Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LILRA5/CD85f protein
Gly42-Arg268, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly42

Predicted Molecular Mass

26 kDa

SDS-PAGE

35-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRA5/CD85f is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 0.1-0.6 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 8956-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRA5/CD85f/LIR-9 Protein, CF

Recombinant Human LILRA5/CD85f/LIR-9 Protein Binding Activity

Recombinant Human LILRA5/CD85f/LIR-9 Protein Binding Activity

When Recombinant Human LILRA5/CD85f is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.1-0.6 µg/mL.

Formulation, Preparation, and Storage

8956-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA5/CD85f

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). Human LILRA5, also known as ILT11, LIR-9, and CD85f, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail (2). The ECD contains two Ig-like domains (3), and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain (4). Alternative splicing generates additional isoforms with a 12 aa deletion within the signal peptide and/or a 27 aa substitution for the C-terminal 61 residues. This substitution encompasses both the transmembrane and cytoplasmic regions. LILRA5 is expressed by monocytes, macrophages, and neutrophils (2, 4). It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients (2, 4). Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10 (2, 4). R&D Systems in-house testing indicates that LILRA5 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (5).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (2003) Blood 101:1484.
  3. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
  4. Mitchell, A. et al. (2008) Eur. J. Immunol. 38:3459.
  5. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Alternate Names

CD85f, ILT11, LILRB7, LIR9

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Gene Symbol

LILRA5

UniProt

A6NI73 (Human)

Additional LILRA5/CD85f Products

Product Documents for Recombinant Human LILRA5/CD85f/LIR-9 Protein, CF

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Product Specific Notices for Recombinant Human LILRA5/CD85f/LIR-9 Protein, CF

For research use only

Customer Reviews for Recombinant Human LILRA5/CD85f/LIR-9 Protein, CF (1)

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/06/2020
    Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)
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