Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF Summary
Accession # Q8NHL6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog #2017-T2) supports the adhesion of HSB2 humanperipheral blood acute lymphoblastic leukemia cells. Immobilized RecombinantHuman LILRB1/CD85j/ILT2 Fc Chimera at 5 µg/mL, 100 µL/well can support 60-80% HSB2cell adhesion, when 1 x 105 cells were added in each well of a 96-well plate.
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HC-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems
in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).
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- Saverino, D. et al. (2000) J. Immunol. 165:3742.
- Saverino, D. et al. (2002) J. Immunol. 168:207.
Citations for Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
Nat Commun, 2016;7(0):11473.
Sample Types: Recombinant Protein
Cis association of leukocyte Ig-like receptor 1 with MHC class I modulates accessibility to antibodies and HCMV UL18.
Authors: Li N, Fu L, Uchtenhagen H, Achour A, Burshtyn D
Eur J Immunol, 2013;43(4):1042-52.
Sample Types: Protein
HLA-G inhibition of NK-cell cytolytic function is uncoupled from tumor cell lipid raft reorganization.
Authors: Baudhuin J, Lesport E, Sousa S, Migraine J, Vigneron J, LeMaoult J, Carosella ED, Mooney N, Favier B
Eur. J. Immunol., 2012;42(3):700-9.
Sample Types: Whole Cells
Applications: Flow Cytometry
HLA-G expression in human embryonic stem cells and preimplantation embryos.
Authors: Verloes A, Van de Velde H, LeMaoult J
J. Immunol., 2011;186(4):2663-71.
Sample Types: Protein
Applications: Luminex Detection
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