Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF

  (4 citations)     
Datasheet / CoA / SDS
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera at 5 µg/mL, 100 µL/well can support 60-80% HSB2 cell adhesion, when 1 x 105 cells were added in each well of a 96-well plate.
  • Source
    Mouse myeloma cell line, NS0-derived human LILRB1/CD85j/ILT2 protein
    Human LILRB1/CD85j/ILT2
    (Gly24-His458)
    Accession # Q8NHL6
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly24
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    73.8 kDa (monomer)
  • SDS-PAGE
    106 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

2017-T2
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: LILRB1/CD85j/ILT2
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HC-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems
in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).
  • References:
    1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
    2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
    3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
    4. Harly, C. et al. (2011) Blood 117:2864.
    5. Cosman, D. et al. (1997) Immunity 7:273.
    6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
    7. Young, N.T. et al. (2008) Blood 111:3090.
    8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
    9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
    10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
    11. Ince, M.N. et al. (2004) Immunology 112:531.
    12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
    13. Saverino, D. et al. (2002) J. Immunol. 168:207.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1
  • Entrez Gene IDs:
    10859 (Human)
  • Alternate Names:
    CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016;7(0):11473.
    Species: Human
    Sample Type: Recombinant Protein
  2. Cis association of leukocyte Ig-like receptor 1 with MHC class I modulates accessibility to antibodies and HCMV UL18.
    Authors: Li N, Fu L, Uchtenhagen H, Achour A, Burshtyn D
    Eur J Immunol, 2013;43(4):1042-52.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  3. HLA-G inhibition of NK-cell cytolytic function is uncoupled from tumor cell lipid raft reorganization.
    Authors: Baudhuin J, Lesport E, Sousa S, Migraine J, Vigneron J, LeMaoult J, Carosella ED, Mooney N, Favier B
    Eur. J. Immunol., 2012;42(3):700-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. HLA-G expression in human embryonic stem cells and preimplantation embryos.
    Authors: Verloes A, Van de Velde H, LeMaoult J
    J. Immunol., 2011;186(4):2663-71.
    Species: Human
    Sample Type: Protein
    Application: Luminex Detection

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

Human IgG1 Antibody

WB , ELISA MAB9894  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Human IgG1 Antibody

WB MAB98941  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human IgG1 Fc Protein, CF

BA 110-HG 72
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee