Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF

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Product Details
Citations (4)
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Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF Summary

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera at 5 µg/mL, 100 µL/well can support 60-80% HSB2 cell adhesion, when 1 x 105 cells were added in each well of a 96-well plate.
Mouse myeloma cell line, NS0-derived human LILRB1/CD85j/ILT2 protein
Human LILRB1/CD85j/ILT2
Accession # Q8NHL6
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
73.8 kDa (monomer)
106 kDa, reducing conditions

Product Datasheets


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HC-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems
in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
  3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
  4. Harly, C. et al. (2011) Blood 117:2864.
  5. Cosman, D. et al. (1997) Immunity 7:273.
  6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
  7. Young, N.T. et al. (2008) Blood 111:3090.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  11. Ince, M.N. et al. (2004) Immunology 112:531.
  12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
  13. Saverino, D. et al. (2002) J. Immunol. 168:207.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1
Entrez Gene IDs
10859 (Human)
Alternate Names
CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7

Citations for Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016;7(0):11473.
    Species: Human
    Sample Types: Recombinant Protein
  2. Cis association of leukocyte Ig-like receptor 1 with MHC class I modulates accessibility to antibodies and HCMV UL18.
    Authors: Li N, Fu L, Uchtenhagen H, Achour A, Burshtyn D
    Eur J Immunol, 2013;43(4):1042-52.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  3. HLA-G inhibition of NK-cell cytolytic function is uncoupled from tumor cell lipid raft reorganization.
    Authors: Baudhuin J, Lesport E, Sousa S, Migraine J, Vigneron J, LeMaoult J, Carosella ED, Mooney N, Favier B
    Eur. J. Immunol., 2012;42(3):700-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. HLA-G expression in human embryonic stem cells and preimplantation embryos.
    Authors: Verloes A, Van de Velde H, LeMaoult J
    J. Immunol., 2011;186(4):2663-71.
    Species: Human
    Sample Types: Protein
    Applications: Luminex Detection


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