Recombinant Human LRRC32/GARP Protein, CF Summary
Met1-Asn627, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with trehalose..|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature human LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine‑rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2, 3). Within the ECD, human LRRC32 shares approximately 80% aa sequence identity with mouse and rat LRRC32. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Human LRRC32 is identified as a lineage specific key receptor for human T cells. It is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6 ‑ 8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
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- Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
- Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
- Macaulay, I.C. et al. (2007) Blood 109:3260.
- Wang, R. et al. (2008) PloS ONE 3:e2705.
- Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
- Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
- Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
- Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
- Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.
Citation for Recombinant Human LRRC32/GARP Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
GARP Regulates the Immune Capacity of a Human Autologous Platelet Concentrate
Authors: ER Trzeciak, N Zimmer, PW Kämmerer, D Thiem, B Al-Nawas, A Tuettenber, S Blatt
Sample Types: Cell Lysates
Applications: Western Blot
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