Recombinant Human LRRC32/GARP Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human LRRC32/GARP is immobilized at 5 μg/mL (100 μL/well) Recombinant Human Latent TGF‑ beta 1 (Catalog # 299-LT) binds with an apparent Kd <30 nM.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Met1-Asn627, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala18
  • Predicted Molecular Mass
    67.0 kDa (monomer)
  • SDS-PAGE
    70-80 kDa, reducing conditions
6055-LR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose..
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC32/GARP
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature human LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine‑rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2, 3). Within the ECD, human LRRC32 shares approximately 80% aa sequence identity with mouse and rat LRRC32. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Human LRRC32 is identified as a lineage specific key receptor for human T cells. It is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6 ‑ 8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
  • References:
    1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
    2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
    3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
    4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
    5. Macaulay, I.C. et al. (2007) Blood 109:3260.
    6. Wang, R. et al. (2008) PloS ONE 3:e2705.
    7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
    8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
    9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
    10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
    11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.
  • Long Name:
    Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
  • Entrez Gene IDs:
    2615 (Human); 434215 (Mouse)
  • Alternate Names:
    D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32
Related Research Areas
Primary Antibodies
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human Latent TGF-beta 1 Protein, CF

BA 299-LT/CF 10  
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Recombinant Human Latent TGF-beta 1 Protein

BA 299-LT 10  
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