Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

(4 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
  • Source
    E. coli-derived Leu35-Leu205, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    19 kDa
  • SDS-PAGE
    17-19 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
211-TBB
 
211-TBB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 4-20 pg/mL.
1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.
Background: Lymphotoxin-alpha/TNF-beta

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).

  • References:
    1. Nedwin, G.E. et al. (1985) J. Cell. Biochem. 29:171.
    2. Gray, P.W. et al. (1984) Nature 312:721.
    3. Kobayashi, Y. et al. (1986) J. Biochem. 100:727.
    4. Warzocha, K. et al. (1995) Eur. Cytokine Netw. 6:83.
    5. McCarthy, D.D. et al.(2006) Immunol. Res. 35:41.
    6. Drutskaya, M.S. et al. (2010) IUBMB Life 62:283.
    7. De Togni, P. et al. (1994) Science 264:703.
    8. Seleznik, G.M. et al. (2014) Cytokine Growth Factor Rev. 25:125.
    9. Mapara, M.Y. et al. (1994) Int. J. Cancer 58:248.
    10. Chiang, E.Y. et al. (2009) Nat. Med. 15:766.
  • Long Name:
    Tumor Necrosis Factor beta
  • Entrez Gene IDs:
    4049 (Human); 16992 (Mouse)
  • Alternate Names:
    LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
    Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
    J Biol Chem, 2015;290(7):4022-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta.
    Authors: Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A
    Eur J Immunol, 2015;45(6):1783-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014;184(4):1152-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
    Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
    Biochem J, 2013;456(3):463-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

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