Recombinant Human LYVE-1 Protein, CF

Catalog # Availability Size / Price Qty
2089-LY-025
Product Details
Citations (1)
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Recombinant Human LYVE-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind hyaluronan in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human LYVE-1 protein
Ser24-Thr238, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser24
Predicted Molecular Mass
24 kDa
SDS-PAGE
40-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2089-LY

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 - 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 - 11).

References
  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
  7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

Citation for Recombinant Human LYVE-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells
    Authors: EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee
    Oncotarget, 2017;8(1):1438-1448.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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