Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
Supplemental Products

Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of C2C12 mouse myoblast cells stimulated for 36 hours with 10% equine serum. When 3 x 104 cells/well are added to rhM-CAD/Fc Chimera coated plates (6 µg/mL, 100 µL/well), approximately 35-60% will adhere after 1 hour at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
Mouse myeloma cell line, NS0-derived human M-Cadherin/Cadherin-15 protein
Human M-CAD
(Val22 - Ala606)
Accession # P55291
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
90.5 kDa (monomer)
122 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: M-Cadherin/Cadherin-15

M-Cadherin (M-CAD or Cadherin-15) is a 124 kDa type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules (1 - 4). Like other classical Cadherins, the 814 amino acid (aa) human M-CAD contains a signal sequence (21 aa), a propeptide (29 aa), an extracellular domain with five Cadherin domain repeats (ECD, 556 aa), a transmembrane segment (20 aa) and a cytoplasmic domain (188 aa) (1). A splice variant that diverges within the third Cadherin repeat has been sequenced. The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells (1 - 4). Intracellularly, M-CAD binds beta -catenin or plakoglobin ( gamma -catenin), which in turn bind alpha -catenin (4). M-CAD also binds p120 catenin (5). Connection of Cadherin/catenin complexes to the actin cytoskeleton is in question, but is possible through a linker (6). Connection to microtubules has been shown (7). M-CAD is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion (8). It is also present in muscle satellite cells and participates in muscle regeneration (9). M-CAD is also expressed in the granule cell layer of the cerebellar glomerulus (10). Deletion of mouse M-CAD has little effect in vivo, most likely due to compensation by N-Cadherin (11). However, M-CAD upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion (8, 12, 13). M-CAD activity is later downregulated by sequestering to caveoli, p120 catenin/RhoA-induced ubiquitination and/or cleavage by calpain-3. This terminates fusion and allows sarcomere formation (5, 12, 13). Human M-CAD ECD shows 88% aa identity with mouse, rat or bovine, and 85% aa identity with canine M-CAD ECD. M-CAD is an outlier among classical Cadherins, with 40% aa identity or less in the ECD (4).

  1. Kaufmann, U. et al. (1999) Cell Tissue Res. 296:191.
  2. Angst, B. D. et al. (2001) J. Cell Sci. 114:629.
  3. Shimoyama, Y. et al. (1998) J. Biol. Chem. 273:10011.
  4. Kuch, C. et al. (1997) Exp. Cell Res. 232:331.
  5. Charasse, S. et al. (2006) Mol. Biol. Cell 17:749.
  6. Weis, W. I. and W. J. Nelson (2006) J. Biol. Chem. 281:35593.
  7. Kaufmann, U. et al. (1999) J. Cell Sci. 112:55.
  8. Zeschnigk, M. et al. (1995) J. Cell Sci. 108:2973.
  9. Wrobel, E. et al. (2007) Eur. J. Cell Biol. Jan 10; [Epub ahead of print]
  10. Rose, O. et al. (1995) Proc. Natl. Acad. Sci. USA 92:6022.
  11. Hollnagel, A. et al. (2002) Mol. Cell. Biol. 22:4760.
  12. Volonte, D. et al. (2003) Mol. Biol. Cell 14:4075.
  13. Kramerova, I. et al. (2006) Mol. Cell. Biol. 26:8437.
Long Name
M-Cadherin [Myotubule]
Entrez Gene IDs
1013 (Human); 12555 (Mouse); 361432 (Rat)
Alternate Names
cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin

Citation for Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 0;21(5):467-75.


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF

There are currently no reviews for this product. Be the first to review Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF and earn rewards!

Have you used Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review