Recombinant Human Meteorin-like/METRNL Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce STAT3 phosphorylation in E16-E18 embryonic rat cortical neurons. 5 μg/mL of Recombinant Human Meteorin-like/METRNL protein significantly induces phosphorylation of STAT3.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human Meteorin-like/METRNL
    (Gln46-Asp311)
    Accession # Q641Q3
    HPGGGSGGGSGGGSHHHHHH
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln46 inferred from enzymatic pyroglutamate treatment revealing Tyr47
  • Predicted Molecular Mass
    32 kDa
9339-MN
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 14 days, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Meteorin-like/METRNL Induces Stat3 Phosphorylation in E16-E18 RatCortical Neurons. Treatments of E16-E18 rat cortical neuronswith 1 μg/ml (1) and 5 μg/ml of Recombinant Human Meteorin-like/METRNL (2) (Catalog #9339-MN) for 15 mintues results in Stat3 phosphorlation. Untreated E16-E18 rat cortical neurons (3).
Background: Meteorin-like/METRNL
Meteorin-like protein (METRNL, Subfatin, Cometin and Interleukin 39) is a 311 amino acid (aa) protein that shows 58% similarity with Meteorin (in human). Human Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide with the secreted mature protein ~30 kDa (1). Mature human Meteorin‑like protein shares 79% and 80% aa sequence identity with mouse and rat METRNL, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide (1). Metrnl is expressed in various adult mouse tissues with the highest expression in muscle tissue, adipose tissue, and activated monocytes (2, 3). Its expression is induced in muscle after exercise and in adipose tissue upon cold exposure suggesting that this secreted protein is both a myokine and an adipokine (4). Neurotrophic activity has been ascribed to Metrnl as it enhances neurite outgrowth and neuroblast migration in vivo. This effect is mediated by the Jak-STAT3 and MEK-ERK pathways (5). Metrnl has also been shown to be indirectly involved in the "browning" of white fat to a more thermogenic "beige fat" through activation of the STAT6 pathway in adipose tissue macrophages, inducing the M2-regulatory cytokines IL-4 and IL-13 (6). Metrnl transgenic mice have demonstrated a role for Metrnl in insulin sensitization through up-regulation of PPAR gamma, a key transcription factor regulating adipocyte differentiation. PPAR gamma increases adipocyte differentiation, inhibits adipose inflammation, activates lipid metabolism, and sensitizes adipocytes to insulin (4).
  • References:
    1. Accession # Q641Q3.
    2. Li, ZY et al. (2014) CNS Neurosci Ther. 20:344.
    3. Ushach, I et al. (2015) Clin Immunol. 156:119.
    4. Zheng, S. et al. (2016) Acta Pharmacol Sin. 37:571.
    5. Jorgensen, J.R. et al. (2012) Exp Neurol. 233:172.
    6. Rao, RR. et al. (2014) Cell. 157:1279.
  • Entrez Gene IDs:
    284207 (Human); 210029 (Mouse); 316842 (Rat)
  • Alternate Names:
    Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human Meteorin-like/METRNL Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information