>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce STAT3 phosphorylation in E16-E18 embryonic rat cortical neurons. 5 μg/mL of Recombinant Human Meteorin-like/METRNL protein significantly induces phosphorylation of STAT3.
Chinese Hamster Ovary cell line, CHO-derived
Human Meteorin-like/METRNL (Gln46-Asp311) Accession # Q641Q3
Recombinant Human Meteorin-like/METRNL Induces Stat3 Phosphorylation in E16-E18 RatCortical Neurons. Treatments of E16-E18 rat cortical neuronswith 1 μg/ml (1) and 5 μg/ml of Recombinant Human Meteorin-like/METRNL (2) (Catalog #9339-MN) for 15 mintues results in Stat3 phosphorlation. Untreated E16-E18 rat cortical neurons (3).
protein (METRNL, Subfatin, Cometin
and Interleukin 39)
is a 311 amino acid (aa) protein that shows 58% similarity with Meteorin (in
human). Human Meteorin‑like protein is synthesized with a 45 amino acid (aa)
signal peptide with the secreted mature protein ~30 kDa (1). Mature human
Meteorin‑like protein shares 79% and 80% aa sequence identity with mouse and
rat METRNL, respectively. Alternate splicing generates an additional isoform
that lacks the N‑terminal 82 amino acids including the signal peptide (1).
Metrnl is expressed in various adult mouse
tissues with the highest expression in muscle tissue, adipose tissue, and
activated monocytes (2, 3). Its expression is induced in muscle after exercise
and in adipose tissue upon cold exposure suggesting that this secreted protein
is both a myokine and an adipokine (4).
Neurotrophic activity has been ascribed to Metrnl as it enhances neurite
outgrowth and neuroblast migration in
vivo. This effect is mediated by the
Jak-STAT3 and MEK-ERK pathways (5).
Metrnl has also been shown to be indirectly involved in the "browning"
of white fat to a more thermogenic "beige fat" through activation of the STAT6
pathway in adipose tissue macrophages, inducing the M2-regulatory cytokines
IL-4 and IL-13 (6). Metrnl transgenic
mice have demonstrated a role for Metrnl in insulin sensitization through
up-regulation of PPAR gamma, a key transcription factor regulating
adipocyte differentiation. PPAR gamma increases adipocyte differentiation, inhibits
adipose inflammation, activates lipid metabolism, and sensitizes adipocytes to
Accession # Q641Q3.
Li, ZY et al. (2014) CNS Neurosci Ther. 20:344.
Ushach, I et al. (2015) Clin Immunol. 156:119.
Zheng, S. et al. (2016) Acta Pharmacol Sin. 37:571.
Jorgensen, J.R. et al. (2012) Exp Neurol. 233:172.
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