Recombinant Human mGluR7 Protein, CF

Catalog # Availability Size / Price Qty
9530-UR-100
The Innovator Series.
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Recombinant Human mGluR7 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human mGluR7 protein
Arg33 & Gln35-Ser521, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg33; Gln35 inferred from enzymatic pyroglutamate treatment revealing Glu36
Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass
55 kDa
SDS-PAGE
55-68 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9530-UR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR7

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulated by phosphorylation, and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). Mature human mGluR8 is 875 amino acids in length, including a 550 amino acid (aa) N-terminal ECD (4). Within N-terminal ECD, human mGluR8 shares 98.4% and 98.5% aa sequence identity with mouse and rat mGluR8, respectively.

References
  1. Conn P.J. and J.P. Pin (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Niswender C.M. and P.J. Conn (2010) Annu. Rev. Pharmacol. Toxicol. 50:295.
  3. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
  4. Makoff A. et al. (1996) Brain Res. Mol. Brain. Res. 40:165.
Long Name
Metabotropic Glutamate Receptor 7
Entrez Gene IDs
2917 (Human); 108073 (Mouse); 81672 (Rat)
Alternate Names
FLJ40498; glutamate receptor, metabotropic 7; GPRC1G; GRM7; metabotropic glutamate receptor 7; MGLU7; mGluR7; MGLUR7GPRC1GGLUR7

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