Recombinant Human MICA His-tag Protein, CF

R&D Systems | Catalog # 10153-MA

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human MICA His-tag Protein (10153-MA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q29983

Applications

Bioactivity
View all MICA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MICA protein
Ala23-Gln308, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

34 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human MICA His-tag (Catalog # 10153-MA) that produces 50% of the optimal binding response is 0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Human MICA His-tag Protein, CF

Recombinant Human MICA His-tag Protein Binding Activity

Recombinant Human MICA His-tag Protein Binding Activity

When Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1.0 µg/mL, 100 µL/well, the concentration of Recombinant MICA His-tag (Catalog # 10153-MA) that produces 50% of the optimal binding response is approximately 0.1-0.5 µg/mL.
Recombinant Human MICA His-tag Protein SDS-PAGE

Recombinant Human MICA His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human MICA His-tag (Catalog # 10153-MA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation, and Storage

10153-MA
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCL with trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICA

Major Histocompatibility Complex Class I Chain-related Gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICA, along with MICB, which shares 91% sequence identity (2), are members of the non-classical MHC class I family. The domain structure of MICA is similar to classical MHC class I proteins: three extracellular Ig-like domains ( alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). Despite structural similarities, MICA shares only 27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and these allow MICA to be reconfigured and bind with NKG2D rather than binding with beta 2-microglobulin (4, 5). MICA genes occur in most mammalian species, but are absent from mouse and rat (2, 4). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 (2, 3). MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta  T cells, and CD8+ alpha beta T cells (6). Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells (4, 7). MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases (8).

References

  1. Huang, C. et al. (2018) Sci. Rep. 8:15821.
  2. Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Stephens, H. A. F. et al. (2001) Trends in Immunology 22:378.
  5. Groh, V. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12445.
  6. Bauer, S. et al. (1999) Science 285:727.
  7. Kawabata, Y. et al. (2000). Human Immunology. 61: 624.
  8. Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.

Long Name

MHC Class I-related Protein A

Alternate Names

FLJ60820, MGC111087, PERB11.1

Entrez Gene IDs

100507436 (Human)

Gene Symbol

MICA

UniProt

Q29983

Additional MICA Products

Product Documents for Recombinant Human MICA His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human MICA His-tag Protein, CF

For research use only

Related Research Areas

Natural Killer T (NKT) Cells

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