Recombinant Human MICA His-tag Protein, CF

Catalog # Availability Size / Price Qty
10153-MA-050
Recombinant Human MICA His-tag Protein, CF Binding Activity
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Recombinant Human MICA His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human MICA His-tag (Catalog # 10153-MA) that produces 50% of the optimal binding response is 0.1-0.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human MICA protein
Ala23-Gln308, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala23
Predicted Molecular Mass
34 kDa
SDS-PAGE
50-70 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10153-MA

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCL with trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1.0 µg/mL, 100 µL/well, the concentration of Recombinant MICA His-tag (Catalog # 10153-MA) that produces 50% of the optimal binding response is approximately 0.1-0.5 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human MICA His-tag (Catalog # 10153-MA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICA

Major Histocompatibility Complex Class I Chain-related Gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICA, along with MICB, which shares 91% sequence identity (2), are members of the non-classical MHC class I family. The domain structure of MICA is similar to classical MHC class I proteins: three extracellular Ig-like domains ( alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). Despite structural similarities, MICA shares only 27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and these allow MICA to be reconfigured and bind with NKG2D rather than binding with beta 2-microglobulin (4, 5). MICA genes occur in most mammalian species, but are absent from mouse and rat (2, 4). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 (2, 3). MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells (6). Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells (4, 7). MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases (8).

References
  1. Huang, C. et al. (2018) Sci. Rep. 8:15821.
  2. Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Stephens, H. A. F. et al. (2001) Trends in Immunology 22:378.
  5. Groh, V. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12445.
  6. Bauer, S. et al. (1999) Science 285:727.
  7. Kawabata, Y. et al. (2000). Human Immunology. 61: 624.
  8. Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

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