Recombinant Human MIS RII Fc Chimera Protein, CF

R&D Systems | Catalog # 4749-MR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human MIS RII Fc Chimera Protein (4749-MR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q16671

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all MIS RII Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MIS RII protein
Human MIS RII
(Pro18 - Ser144)
Accession # Q16671		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro18

Predicted Molecular Mass

40.2 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to bind rhMIS (Catalog # 1737-MS) in a functional ELISA.

Reviewed Applications

Read 2 reviews rated 5 using 4749-MR in the following applications:

Formulation, Preparation, and Storage

4749-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIS RII

Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82 kDa serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-beta superfamily (1). The MIS RII precursor is 573 amino acids (aa) in length, with a 17 aa signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1 - 6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1 - 6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6).

References

  1. Imbeaud, S. et al. (1995) Nat. Genet. 11:382.
  2. Baarends, W.M. et al. (1994) Development 120:189.
  3. di Clemente, N. et al. (1994) Mol. Endocrinol. 8:1006.
  4. Teixeira, J. et al. (1996) Endocrinology 137:160.
  5. Salhi, I et al. (2004) Biochem. J. 379:785.
  6. Imbeaud, S. et al. (1996) Hum. Mol. Genet. 5:1269.

Long Name

Mullerian-Inhibiting Substance Type II Receptor

Alternate Names

AMHR2, AMHRII, C14, MISR2, MISRII

Entrez Gene IDs

269 (Human); 110542 (Mouse); 29530 (Rat)

Gene Symbol

AMHR2

UniProt

Q16671

Additional MIS RII Products

Product Documents for Recombinant Human MIS RII Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human MIS RII Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human MIS RII Fc Chimera Protein, CF

Customer Reviews for Recombinant Human MIS RII Fc Chimera Protein, CF (2)

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  • Recombinant Human MIS RII Fc Chimera Protein, CF
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    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/06/2018
    Recombinant Human MIS RII Fc Chimera Protein, CF 4749-MR
  • Recombinant Human MIS RII Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/30/2018
    Recombinant Human MIS RII Fc Chimera Protein, CF 4749-MR

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