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Recombinant Human MIS/AMH Protein

R&D Systems | Catalog # 1737-MS

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human MIS/AMH Protein (1737-MS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q6GTN3

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all MIS/AMH Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MIS/AMH protein
Ala453-Arg560

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala453

Predicted Molecular Mass

11.7 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human MIS/AMH at 3 µg/mL (100 µL/well) will bind Recombinant Rat MIS RII Fc Chimera (Catalog # 1618-MR) with a linear range of 1.6-100 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1737-MS
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1737-MS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIS/AMH

Müllerian inhibiting substance (MIS), also named anti‑Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1-24), a pro‑region (25-455), and the carboxyl-terminal bioactive protein (446-553) (2‑4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro‑protein. Proteolytic cleavage is required to generate the N-terminal pro‑region and the C‑terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C‑terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro‑region showed enhanced activity (3, 5). The C‑terminal region contains the seven canonical cysteine residues found in TGF-beta  superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro‑region and C‑terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti‑proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10 - 12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10‑12).

References

  1. Teixeira, et al. (2001) Endocrine Rev. 22:657.
  2. Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
  3. Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
  4. Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
  5. Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. 93:7711.
  6. MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
  7. Laurich, V.M. et al. (2002) Endocrinology 143:3351.
  8. McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
  9. Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. 99:239.
  10. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
  11. Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
  12. Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Alternate Names

AMH

Entrez Gene IDs

268 (Human); 11705 (Mouse)

Gene Symbol

AMH

UniProt

Q6GTN3

Additional MIS/AMH Products

Product Documents for Recombinant Human MIS/AMH Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1737-MS (with carrier)
Download SDS 1737-MS/CF (carrier free)

Product Specific Notices for Recombinant Human MIS/AMH Protein

For research use only

Citations for Recombinant Human MIS/AMH Protein

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FAQs for Recombinant Human MIS/AMH Protein

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  • Q: Is Recombinant Human MIS/AMH Protein (Catalog # 1737-MS) the full length or cleaved length version on this protein?

    A: Natural Human MIS is expressed as a proform that is 560 aa long, with a cleavage site between aa450-451. The C-terminal region is the bioactive region. Catalog # 1737-MS consists of Ala453-Arg560, which is the C-terminal bioactive region.

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